Substrates: the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis Products: -
Substrates: Vmax/Km for the synthesis of beta-citrylglutamate is 4fold higher than the Vmax/Km value for the synthesis of N-acetylaspartyl-glutamate Products: -
Substrates: the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis Products: -
from the Vmax, kcat values of 3 and 1.5/s are calculated for the beta-citrylglutamate synthase activity and and N-acetylaspartyl-glutamate synthase activity
the rates for beta-citrylglutamate synthesis and N-acetylaspartyl-glutamate synthesis are similar between pH 7 and 8, but the beta-citrylglutamate synthetase is severalfold higher than the N-acetylaspartyl-glutamate synthetase activity at more alkaline pH
enzyme deficiency leads to male subfertility. Enzyme-deficient mice are impaired in a late step of spermiogenesis and produce spermatozoa with abnormally shaped heads and nuclei. Sperm chromatin in enzyme-deficient mice is less condensed and showed impaired histone to protamine exchange and retained transition protein 2
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
produced it in Escherichia coli either unmodified or as a fusion protein with a poly-His tag at the C terminus. Extracts of cells expressing these recombinant proteins display N-acetylaspartylglutamate synthase activity
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