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Information on EC 6.3.2.38 - N2-citryl-N6-acetyl-N6-hydroxylysine synthase and Organism(s) Escherichia coli and UniProt Accession Q47316

for references in articles please use BRENDA:EC6.3.2.38
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IUBMB Comments
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme is believed to form an adenylate intermediate prior to ligation.
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This record set is specific for:
Escherichia coli
UNIPROT: Q47316
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The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Synonyms
aerD, EC 6.3.2.27, iuacA, iucA, Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase
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SYSTEMATIC NAME
IUBMB Comments
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming)
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme is believed to form an adenylate intermediate prior to ligation.
CAS REGISTRY NUMBER
COMMENTARY hide
94047-30-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O
2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine
show the reaction diagram
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-
-
?
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O
2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O
2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine
show the reaction diagram
-
-
-
?
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O
2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IUCA_ECOLX
575
1
65570
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lorenzo de, V.; Bindereif, A.; Paw, B.H.; Neilands, J.B.
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12
J. Bacteriol.
165
570-578
1986
Escherichia coli (Q47316)
Manually annotated by BRENDA team
Challis, G.L.
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases
ChemBioChem
6
601-611
2005
Escherichia coli
Manually annotated by BRENDA team
Gross, R.; Engelbrecht, F.; Braun, V.
Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids
Mol. Gen. Genet.
201
204-212
1985
Escherichia coli
Manually annotated by BRENDA team