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Information on EC 6.3.2.1 - pantoate-beta-alanine ligase (AMP-forming) and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FKB3

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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FKB3 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
synthetase, pantothenate, pantothenate synthetase, mtbps, pantoate-beta-alanine ligase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Pantothenate synthetase
-
D-Pantoate:beta-alanine ligase (AMP-forming)
-
-
-
-
Pantoate activating enzyme
-
-
-
-
pantoate-beta-alanine ligase
-
-
-
-
Pantoic-activating enzyme
-
-
-
-
Pantothenate synthetase
-
-
-
-
Synthetase, pantothenate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide formation
-
-
-
-
carboxamide formation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-pantoate:beta-alanine ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-49-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
-
-
-
?
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
-
-
-
?
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-pantoate
-
substrate inhibition
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.039 - 0.81
(R)-pantoate
0.16 - 0.59
beta-Alanine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
exchange between beta-alanine and pantothenate in the presence of AMP
9
-
synthesis of pantothenate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Columbia-0, single gene
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcript and metabolite patterns of CoA biosynthesis during seed development involving the enzyme, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PANC_ARATH
310
0
34137
Swiss-Prot
other Location (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E132A
-
kcat for beta-alanine is 3.7fold higher than wild-type value, kcat for pantoate is 21fold higher than wild-type value
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
promoter:beta-glucuronidase analysis
expression in Escherichia coli as N-terminal His-tagged protein
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ottenhof, H.H.; Ashurst, J.L.; Whitney, H.M.; Saldanha, S.A.; Schmitzberger, F.; Gweon, H.S.; Blundell, T.L.; Abell, C.; Smith, A.G.
Organisation of the pantothenate (vitamin B5) biosynthesis pathway in higher plants
Plant J.
37
61-72
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Jonczyk, R.; Genschel, U.
Molecular adaptation and allostery in plant pantothenate synthetases
J. Biol. Chem.
281
37435-37446
2006
Arabidopsis thaliana, Escherichia coli
Manually annotated by BRENDA team
Jonczyk, R.; Ronconi, S.; Rychlik, M.; Genschel, U.
Pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis
Plant Mol. Biol.
66
1-14
2008
Escherichia coli, Arabidopsis thaliana (Q9FKB3), Arabidopsis thaliana
Manually annotated by BRENDA team