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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms theanine synthetase, glutamate-ethylamine ligase, more
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N5-Ethyl-L-glutamine synthetase
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Synthetase, N5-ethylglutamine
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Theanine synthetase
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ATP + L-glutamate + ethylamine = ADP + phosphate + N5-ethyl-L-glutamine
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Acid amide hydrolysis
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carboxylic
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L-glutamate:ethylamine ligase (ADP-forming)
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ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
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Substrates: - Products: -
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ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
DD410895
Substrates: - Products: -
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ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
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Substrates: - Products: i.e. theanine
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ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
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Substrates: - Products: -
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ATP + L-glutamate + ethylamine
ADP + phosphate + N5-ethyl-L-glutamine
DD410895
Substrates: - Products: -
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ATP
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Mn2+
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less effective than Mg2+ in activation
NaCl
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theanine biosynthesis, derived from nitrogen metabolism in Camellia sinensis plants, can be influenced by salt treatment
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beta-phenylethylamine
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NH4+
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0.5 mM, complete inhibition
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6.5 - 8.5
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6.5: about 25% of maximal activity, 8.5: about 45% of maximal activity
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brenda
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DD410895
GenBank
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Anhui Agricultural University, Hefei city, Anhui Province, China
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tea
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DD410895
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main and lateral root, highest enzymic activity among all organs tested
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DD410895
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substrates ethylamine and glutamic acid are distributed almost uniformly in all parts of the seedling
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DD410895
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Highest Expressing Human Cell Lines
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physiological function
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the flavor of tea is conferred by certain metabolites, especially L-theanine, in Camellia sinensis. More L-theanine accumulates in Camellia sinensis than in other plants. L-Glutamic acid, a precursor of L-theanine, is present in most plants, while ethylamine, another precursor of L-theanine, specifically accumulates in Camellia species, especially Camellia sinensis. Most plants contain the enzyme/gene, glutamate-ethylamine ligase, catalyzing the conversion of ethylamine and L-glutamic acid to L-theanine. After supplementation with [2H5]ethylamine, all the plants tested (Camellia nitidissima, Camellia japonica, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum) produce [2H5]l-theanine, which suggests that ethylamine availability is the reason for the difference in L-theanine accumulation between Camellia sinensis and other plants
metabolism
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the enzyme is involved in theanine biosynthesis in tea plants. Theanine is the most abundant amino acid in tea seedlings
metabolism
DD410895
theanine synthetase is a key enzyme involved in theanine biosynthesis, theanine biosynthesis is derived from nitrogen metabolism in Camellia sinensis
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GMAS_METMY
444
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48925
Swiss-Prot
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A0A8H9CN36_9PSED
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48943
TrEMBL
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additional information
DD410895
long-term shade treatment induces the enzyme expression, whhich may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions. The expression remains at a high level, overview
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NaCl induces the enzyme expression in planta, levels of theanine and the total free amino acids are gradually increased in shoots, and reach the maximum on the 8th day after treatment
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Sasaoka, K.; Kito, M.; Onishi, Y.
Some properties of the theanine synthesizing enzyme in tea seedlings
Agric. Biol. Chem.
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984-988
1965
Camellia sinensis
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brenda
Sasaoka, K.; Kito, M.
Synthesis of theanine by the homogenate of tea seedlings
Agric. Biol. Chem.
28
313-317
1964
Camellia sinensis
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brenda
Deng, W.W.; Ogita, S.; Ashihara, H.
Ethylamine content and theanine biosynthesis in different organs of Camellia sinensis seedlings
Z. Naturforsch. C
64
387-390
2009
Camellia sinensis
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Deng, W.; Fei, Y.; Wang, S.; Wan, X.; Zhang, Z.; Hu, X.
Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings
Plant Growth Regul.
71
295-299
2013
Camellia sinensis (DD410895)
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brenda
Deng, W.W.; Wang, S.; Chen, Q.; Zhang, Z.Z.; Hu, X.Y.
Effect of salt treatment on theanine biosynthesis in Camellia sinensis seedlings
Plant Physiol. Biochem.
56
35-40
2012
Camellia sinensis
brenda
Cheng, S.; Fu, X.; Wang, X.; Liao, Y.; Zeng, L.; Dong, F.; Yang, Z.
Studies on the biochemical formation pathway of the amino acid L-theanine in tea (Camellia sinensis) and other plants
J. Agric. Food Chem.
65
7210-7216
2017
Camellia sinensis
brenda
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