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Information on EC 6.2.1.8 - oxalate-CoA ligase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SMT7

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EC Tree
     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.8 oxalate-CoA ligase
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9SMT7 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
oxalyl-coa synthetase, acyl-activating enzyme 3, acyl activating enzyme3, scaae3, acyl-activating enzyme3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acyl-activating enzyme3
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Oxalyl-CoA synthetase
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OCS
-
-
-
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Oxalate:CoA ligase (AMP)
-
-
-
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Oxalyl CoA synthetase
-
-
-
-
Oxalyl coenzyme A synthetase
-
-
-
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Oxalyl-CoA synthetase
-
-
-
-
Synthetase, oxalyl coenzyme A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
oxalate:CoA ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37318-57-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + oxalate + CoA
AMP + diphosphate + oxalyl-CoA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + oxalate + CoA
AMP + diphosphate + oxalyl-CoA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required in form of Mg-ATP
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04215 - 0.149
oxalate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
activity range, profile overview
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
aae3 null mutants show increased soluble and insoluble oxalate contents compared to the wild-type enzyme, loss of AAE3 interferes with seed development affecting the integrity and function of the seed coat, phenotype, overview
metabolism
the enzyme catalyzes the first step in oxalate degradation
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
4CLLA_ARATH
514
0
55544
Swiss-Prot
other Location (Reliability: 3)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H214A
the mutant shows strongly reduced activity compared to the wild type enzyme
H319A
the mutant shows strongly reduced activity compared to the wild type enzyme
K500A
the mutant shows strongly reduced activity compared to the wild type enzyme
R409A
the mutant shows strongly reduced activity compared to the wild type enzyme
S289A
the mutant shows strongly reduced activity compared to the wild type enzyme
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Foster, J.; Kim, H.U.; Nakata, P.A.; Browse, J.
A previously unknown oxalyl-CoA synthetase is important for oxalate catabolism in Arabidopsis
Plant Cell
24
1217-1229
2012
Arabidopsis thaliana (Q9SMT7), Arabidopsis thaliana
Manually annotated by BRENDA team
Fan, M.; Xiao, Y.; Li, M.; Chang, W.
Crystal structures of Arabidopsis thaliana oxalyl-CoA synthetase essential for oxalate degradation
Mol. Plant
9
1349-1352
2016
Arabidopsis thaliana (Q9SMT7), Arabidopsis thaliana
Manually annotated by BRENDA team