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IUBMB Comments The enzyme, characterized from the bacterium Aromatoleum aromaticum , is involved in degradation of (indol-3-yl)acetate. It is also active with phenylacetate and the non-physiological compound (2-naphthyl)acetate.
The expected taxonomic range for this enzyme is: Aromatoleum aromaticum
Synonyms indoleacetate-coa ligase, more
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iaaB
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ATP + (indol-3-yl)acetate + CoA = AMP + diphosphate + (indol-3-yl)acetyl-CoA
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(indol-3-yl)acetate:CoA ligase (AMP-forming)
The enzyme, characterized from the bacterium Aromatoleum aromaticum, is involved in degradation of (indol-3-yl)acetate. It is also active with phenylacetate and the non-physiological compound (2-naphthyl)acetate.
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ATP + (indol-3-yl)acetate + CoA
AMP + diphosphate + (indol-3-yl)acetyl-CoA
ATP + 2-methyl-indole-3-acetate + CoA
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Substrates: 6.3% activity compared to (indol-3-yl)acetate Products: -
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ATP + 2-naphthyl acetate + CoA
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Substrates: 124% activity compared to (indol-3-yl)acetate Products: -
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ATP + 4-hydroxyphenyl acetate + CoA
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Substrates: 12% activity compared to (indol-3-yl)acetate Products: -
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ATP + indolepropionate + CoA
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Substrates: 2.9% activity compared to (indol-3-yl)acetate Products: -
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ATP + phenyl acetate + CoA
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Substrates: 20% activity compared to (indol-3-yl)acetate Products: -
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additional information
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Substrates: no activity with phenyl succinate, benzyl malonate, succinate, benzyl succinate, indole-3-carboxylate, indolebutyrate or L-tryptophan Products: -
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ATP + (indol-3-yl)acetate + CoA
AMP + diphosphate + (indol-3-yl)acetyl-CoA
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Substrates: - Products: -
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ATP + (indol-3-yl)acetate + CoA
AMP + diphosphate + (indol-3-yl)acetyl-CoA
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Substrates: 100% activity Products: -
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ATP + (indol-3-yl)acetate + CoA
AMP + diphosphate + (indol-3-yl)acetyl-CoA
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Substrates: - Products: -
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(indol-3-yl)acetate
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substrate inhibition
2,4-dichlorophenoxyacetate
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2-naphthyl acetate
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substrate inhibition
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0.035
(indol-3-yl)acetate
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pH and temperature not specified in the publication
0.097
2-naphthyl acetate
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pH and temperature not specified in the publication
0.42
phenyl acetate
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pH and temperature not specified in the publication
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4.3
(indol-3-yl)acetate
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pH and temperature not specified in the publication
4.7
2-naphthyl acetate
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pH and temperature not specified in the publication
0.48
phenyl acetate
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pH and temperature not specified in the publication
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123
(indol-3-yl)acetate
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pH and temperature not specified in the publication
48
2-naphthyl acetate
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pH and temperature not specified in the publication
1
phenyl acetate
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pH and temperature not specified in the publication
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3.9
(indol-3-yl)acetate
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pH and temperature not specified in the publication
6.5
2-naphthyl acetate
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pH and temperature not specified in the publication
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1.6
2,4-dichlorophenoxyacetate
Aromatoleum aromaticum
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pH and temperature not specified in the publication
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IAAB_AROAE
Aromatoleum aromaticum (strain DSM 19018 / LMG 30748 / EbN1)
510
0
55564
Swiss-Prot
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monomer
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1 * 57000, calculated from amino acid sequence
monomer
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1 * 55000, SDS-PAGE
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Strep-tag affinity column chromatography
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expressed in Escherichia coli DH5alpha cells
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Schuehle, K.; Nies, J.; Heider, J.
An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin
Environ. Microbiol.
18
3120-3132
2016
Aromatoleum aromaticum
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