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EC Tree
IUBMB Comments The enzyme, characterized from the marine ammonia-oxidizing archaeon Nitrosopumilus maritimus, participates in a variant of the 3-hydroxypropanoate/4-hydroxybutanate CO2 fixation cycle. cf. EC 6.2.1.40, 4-hydroxybutyrate---CoA ligase (AMP-forming).
The expected taxonomic range for this enzyme is: Nitrosopumilus maritimus
Synonyms
4-hydroxybutyryl-CoA synthetase,
Nmar_0206 ,
more
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4-hydroxybutyryl-CoA synthetase
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Nmar_0206
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locus name
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ATP + 4-hydroxybutanoate + CoA = ADP + phosphate + 4-hydroxybutanoyl-CoA
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4-hydroxybutanoate:CoA ligase (ADP-forming)
The enzyme, characterized from the marine ammonia-oxidizing archaeon Nitrosopumilus maritimus, participates in a variant of the 3-hydroxypropanoate/4-hydroxybutanate CO2 fixation cycle. cf. EC 6.2.1.40, 4-hydroxybutyrate---CoA ligase (AMP-forming).
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ATP + 4-hydroxybutanoate + CoA
ADP + phosphate + 4-hydroxybutanoyl-CoA
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?
ATP + butanoate + CoA
ADP + phosphate + butanoyl-CoA
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?
additional information
?
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very poor substrates: acetate, propionate
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?
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additional information
not activating: Ni2+, Co2+, Ca2+
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0.37
4-hydroxybutanoate
pH not specified in the publication, temperature not specified in the publication
0.22
ATP
pH not specified in the publication, temperature not specified in the publication
5
Butanoate
pH not specified in the publication, temperature not specified in the publication
0.16
CoA
pH not specified in the publication, temperature not specified in the publication
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4.88
4-hydroxybutanoate
pH not specified in the publication, temperature not specified in the publication
9.96
ATP
pH not specified in the publication, temperature not specified in the publication
0.16
Butanoate
pH not specified in the publication, temperature not specified in the publication
11.28
CoA
pH not specified in the publication, temperature not specified in the publication
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20
cell extract, pH not specified in the publication, temperature not specified in the publication
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UniProt
brenda
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physiological function
the enzyme participates in a variant of the 3-hydroxypropanoate/4-hydroxybutanate CO2 fixation cycle
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HBCAL_NITMS
Nitrosopumilus maritimus (strain SCM1)
698
0
75619
Swiss-Prot
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?
x * 76500, calculated from sequence
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expression in Escherichia coli
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Koenneke, M.; Schubert, D.; Brown, P.; Huegler, M.; Standfest, S.; Schwander, T.; Schada Von Borzyskowski, L.; Erb, T.; Stahl, D.; Berg, I.
Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO2 fixation
Proc. Natl. Acad. Sci. USA
111
8239-8244
2014
Nitrosopumilus maritimus (A9A1Y1)
brenda
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