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IUBMB Comments Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L -glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95 , L -glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L -glutamate molecule.
Requires Mg2+ 2+ 2+
Synonyms BtrJ , [BtrI acyl-carrier protein]L-glutamate ligase,
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[BtrI acyl-carrier protein]L-glutamate ligase
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BtrJ
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ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
(2)
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ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
(1)
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MetaCyc
butirosin biosynthesis
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[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
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ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
additional information
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ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
Substrates: -
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ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
Substrates: -
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ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Substrates: -
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ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Substrates: -
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ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
Substrates: BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond
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ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
Substrates: BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond
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ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
Substrates: -
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ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
Substrates: -
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additional information
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Substrates: the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay
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additional information
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Substrates: the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay
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ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Substrates: -
?
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
Substrates: -
?
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
Substrates: -
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ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
Substrates: -
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Mg2+
essentially required
Mn2+
essentially required, activates
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gene btrJ
UniProt
brenda
gene btrJ
UniProt
brenda
Highest Expressing Human Cell Lines
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Gene Links
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evolution
BtrJ belongs to the ATP-grasp superfamily
evolution
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BtrJ belongs to the ATP-grasp superfamily
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metabolism
butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview
metabolism
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butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview
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BTRJ_NIACI
419
0
48648
Swiss-Prot
other Location (Reliability: 2 )
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48600
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50962
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50969
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
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monomer
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
monomer
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1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
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additional information
purified BtrJ shows aggregation in solution, preventable by DTT
additional information
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purified BtrJ shows aggregation in solution, preventable by DTT
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gene btrJ, butirosin genetic cluster organization, overview. Expression of N-terminal His6-tagged BtrJ
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Li, Y.; Llewellyn, N.M.; Giri, R.; Huang, F.; Spencer, J.B.
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway
Chem. Biol.
12
665-675
2005
Niallia circulans (Q4H4E7), Niallia circulans NR3312 (Q4H4E7)
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