Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 6.1.1.5 - isoleucine-tRNA ligase and Organism(s) Pseudomonas fluorescens and UniProt Accession Q8L1B1

for references in articles please use BRENDA:EC6.1.1.5
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas fluorescens
UNIPROT: Q8L1B1 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Pseudomonas fluorescens
The enzyme appears in selected viruses and cellular organisms
Synonyms
isoleucyl-trna synthetase, ilers, iars2, isoleucyl trna synthetase, iles2, ile-trna synthetase, isoleucine-trna synthetase, mitochondrial isoleucyl-trna synthetase, iles1, ecilers, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Isoleucine translase
-
Isoleucine--tRNA ligase
-
Isoleucine-transfer RNA ligase
-
Isoleucine-tRNA synthetase
-
Isoleucyl-transfer ribonucleate synthetase
-
Isoleucyl-transfer RNA synthetase
-
Isoleucyl-tRNA synthetase
-
Mupirocin resistance protein
-
Synthetase, isoleucyl-transfer ribonucleate
-
IleRS
-
-
-
-
IRS
-
-
-
-
Isoleucine translase
-
-
-
-
Isoleucine--tRNA ligase
-
-
-
-
Isoleucine-transfer RNA ligase
-
-
-
-
Isoleucine-tRNA synthetase
-
-
-
-
Isoleucyl-transfer ribonucleate synthetase
-
-
-
-
Isoleucyl-transfer RNA synthetase
-
-
-
-
Isoleucyl-tRNA synthetase
-
-
-
-
Mupirocin resistance protein
-
-
-
-
Synthetase, isoleucyl-transfer ribonucleate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
-
-
aminacylation
-
-
-
-
deacylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
L-isoleucine:tRNAIle ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-96-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-isoleucine + tRNAIle
AMP + diphosphate + L-isoleucyl-tRNAIle
show the reaction diagram
tRNAIle from Pseudomonas fluorescens and Escherichia coli
-
?
ATP + L-isoleucine + tRNAIle
AMP + diphosphate + L-isoleucyl-tRNAIle
show the reaction diagram
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-isoleucine + tRNAIle
AMP + diphosphate + L-isoleucyl-tRNAIle
show the reaction diagram
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pseudomonic acid
i.e. muciproin
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007 - 0.33
ATP
0.00125 - 0.012
L-isoleucine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.64 - 4.63
L-isoleucine
0.18 - 6
L-isoleucine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000105 - 0.000015
pseudomonic acid
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SYI2_PSEFL
1030
0
117875
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 117000-117738, native isozyme IleRS-R2, SDS-PAGE and amino acid sequence determination
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme, and recombinant enzyme from Escherichia coli, amino acid sequence determination
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yanagisawa, T.; Kawakami, M.
How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense
J. Biol. Chem.
278
25887-25894
2003
Pseudomonas fluorescens, Pseudomonas fluorescens (Q8L1B1), Pseudomonas fluorescens NCIB 10586, Pseudomonas fluorescens NCIB 10586 (Q8L1B1)
Manually annotated by BRENDA team