Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 6.1.1.17 - glutamate-tRNA ligase and Organism(s) Methanothermobacter thermautotrophicus and UniProt Accession O26157

for references in articles please use BRENDA:EC6.1.1.17
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Methanothermobacter thermautotrophicus
UNIPROT: O26157 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Methanothermobacter thermautotrophicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
glurs, glutaminyl-trna synthetase, glutamyl-trna synthetase, glurs2, trna modifying enzyme, glurs1, glutamyl trna synthetase, discriminating glurs, glursat, d-glurs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GluRS
Glutamate--tRNA ligase
-
-
-
-
Glutamate-tRNA synthetase
-
-
-
-
Glutamic acid translase
-
-
-
-
Glutamic acid tRNA ligase
-
-
-
-
Glutamyl tRNA synthetase
-
-
-
-
Glutamyl-transfer ribonucleate synthetase
-
-
-
-
Glutamyl-transfer ribonucleic acid synthetase
-
-
-
-
Glutamyl-transfer RNA synthetase
-
-
-
-
Glutamyl-tRNA synthetase
P85
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
-
-
Aminoacylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamate:tRNAGlu ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-76-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + tRNAGln(CUG)
AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
show the reaction diagram
-
the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG)
-
-
?
ATP + L-glutamate + tRNAGln(UUG)
AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
show the reaction diagram
-
the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG)
-
-
?
ATP + L-glutamate + tRNAGlu
AMP + diphosphate + L-glutamyl-tRNAGlu
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + tRNAGln(CUG)
AMP + diphosphate + L-glutamyl-tRNAGln(CUG)
show the reaction diagram
-
the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG)
-
-
?
ATP + L-glutamate + tRNAGln(UUG)
AMP + diphosphate + L-glutamyl-tRNAGln(UUG)
show the reaction diagram
-
the enzyme shows a significant catalytic preference for tRNAGln(CUG) compared to the less active tRNAGln(UUG)
-
-
?
ATP + L-glutamate + tRNAGlu
AMP + diphosphate + L-glutamyl-tRNAGlu
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00133
tRNAGln(CUG)
-
wild type enzyme, pH and temperature not specified in the publication
0.00539
tRNAGln(UUG)
-
wild type enzyme, pH and temperature not specified in the publication
0.00361
tRNAGlu
-
wild type enzyme, pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.041
tRNAGln(CUG)
-
wild type enzyme, pH and temperature not specified in the publication
0.006
tRNAGln(UUG)
-
wild type enzyme, pH and temperature not specified in the publication
0.04
tRNAGlu
-
wild type enzyme, pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
310
tRNAGln(CUG)
-
wild type enzyme, pH and temperature not specified in the publication
1
tRNAGln(UUG)
-
wild type enzyme, pH and temperature not specified in the publication
10
tRNAGlu
-
wild type enzyme, pH and temperature not specified in the publication
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
O'Donoghue, P.; Sheppard, K.; Nureki, O.; Soell, D.
Rational design of an evolutionary precursor of glutaminyl-tRNA synthetase
Proc. Natl. Acad. Sci. USA
108
20485-20490
2011
Methanothermobacter thermautotrophicus
Manually annotated by BRENDA team