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Information on EC 6.1.1.15 - proline-tRNA ligase and Organism(s) Plasmodium falciparum and UniProt Accession Q8I5R7

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Plasmodium falciparum
UNIPROT: Q8I5R7 not found.
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The taxonomic range for the selected organisms is: Plasmodium falciparum
The enzyme appears in selected viruses and cellular organisms
Synonyms
prors, prolyl-trna synthetase, glutamyl-prolyl-trna synthetase, glutamyl-prolyl trna synthetase, gluprors, prolyl trna synthetase, prorstt, procysrs, bifunctional aminoacyl-trna synthetase, glutamyl-/prolyl-trna synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Prolyl-tRNA synthetase
-
Global RNA synthesis factor
-
-
-
-
Pro-tRNA synthetase
-
-
-
-
Proline translase
-
-
-
-
Proline--tRNA ligase
-
-
-
-
Prolyl RNA synthetase
-
-
-
-
Prolyl-transfer ribonucleate synthetase
-
-
-
-
Prolyl-transfer ribonucleic acid synthetase
-
-
-
-
Prolyl-transfer RNA synthetase
-
-
-
-
Prolyl-tRNA synthetase
-
-
-
-
ProRS
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
-
-
Aminoacylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
L-proline:tRNAPro ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9055-68-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 5'-(beta,gamma-imido)triphosphate + L-proline + tRNAPro
?
show the reaction diagram
-
-
-
?
ATP + L-proline + tRNAPro
AMP + diphosphate + L-prolyl-tRNAPro
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-proline + tRNAPro
AMP + diphosphate + L-prolyl-tRNAPro
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-[4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-3-(trifluoromethyl)-1H-pyrazol-1-yl]acetamide
-
3-[4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-1H-pyrazol-3-yl]propanamide
-
4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-amine
-
4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-1H-pyrazole-3-carboxamide
-
6-fluoro-febrifugine
-
7-bromo-6-chloro-3-[3-[3-hydroxy-2-piperidinyl]-2-oxopropyl]-4-quinazolinone
-
halofuginone
N-(2,4-difluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(3-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[1-methyl-3-(trifluoromethyl)-4-[3-(trifluoromethyl)phenyl]-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-(2-hydroxyethyl)-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-[2-(morpholin-4-yl)ethyl]-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-3-(2-hydroxyethyl)-1-methyl-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-[2-(2-hydroxyethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-[2-(hydroxymethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-(4-fluorophenyl)-N'-[4-[3-(2-hydroxyethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
-
N-([3-[5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-4-yl]phenyl]methyl)methanesulfonamide
-
N-Boc-halofuginone
less than 20% inhibition at 1 mM
N-[3-(2-cyanoethyl)-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
N-[3-(cyanomethyl)-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
N-[3-cyano-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
N-[3-[2-(dimethylamino)ethyl]-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
N-[4-(3,4-difluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
N-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-methoxyphenyl)urea
-
N-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-[2-(morpholin-4-yl)pyridin-4-yl]urea
-
N-[4-bromo-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
-
TCMDC-124506
competitive inhibitor
tetrahydrofebrifugine
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
2-[4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-3-(trifluoromethyl)-1H-pyrazol-1-yl]acetamide
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.008
3-[4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-1H-pyrazol-3-yl]propanamide
Plasmodium falciparum
at pH 7.0 and 37°C
0.05
4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-amine
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.014
4-(4-fluorophenyl)-5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-1H-pyrazole-3-carboxamide
Plasmodium falciparum
at pH 7.0 and 37°C
0.000013
6-fluoro-febrifugine
Plasmodium falciparum
at pH 7.4 and 37°C
0.000024
febrifugine
Plasmodium falciparum
at pH 7.4 and 37°C
0.000009
halofuginone
Plasmodium falciparum
at pH 7.4 and 37°C
0.0025
N-(2,4-difluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.008
N-(3-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[1-methyl-3-(trifluoromethyl)-4-[3-(trifluoromethyl)phenyl]-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-(2-hydroxyethyl)-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.005
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.003
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-1-methyl-3-[2-(morpholin-4-yl)ethyl]-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.018
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-3-(2-hydroxyethyl)-1-methyl-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[4-(4-fluorophenyl)-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-(4-fluorophenyl)-N'-[4-[2-(2-hydroxyethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.02
N-(4-fluorophenyl)-N'-[4-[2-(hydroxymethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.013
N-(4-fluorophenyl)-N'-[4-[3-(2-hydroxyethyl)phenyl]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.05
N-([3-[5-[[(4-fluorophenyl)carbamoyl]amino]-1-methyl-3-(trifluoromethyl)-1H-pyrazol-4-yl]phenyl]methyl)methanesulfonamide
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.009
N-[3-(2-cyanoethyl)-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.006
N-[3-(cyanomethyl)-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.005
N-[3-cyano-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.004
N-[3-[2-(dimethylamino)ethyl]-4-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
at pH 7.0 and 37°C
0.05
N-[4-(3,4-difluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-methoxyphenyl)urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-[4-(4-fluorophenyl)-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-[2-(morpholin-4-yl)pyridin-4-yl]urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.05
N-[4-bromo-1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-yl]-N'-(4-fluorophenyl)urea
Plasmodium falciparum
IC50 above 0.05 mM, at pH 7.0 and 37°C
0.005
TCMDC-124506
Plasmodium falciparum
at pH 7.0 and 37°C
0.000352
tetrahydrofebrifugine
Plasmodium falciparum
at pH 7.4 and 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 58000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 2 % (v/v) Tacsimate (pH 5.0), and 12% (w/v) polyethylene glycol 3350
sitting drop vapor diffusion method, using 10% (w/v) PEG-800, 20% (v/v) ethylene glycol, 0.1 M MES/imidazole, pH 6.5, and 0.03 M each sodium fluoride, sodium bromide, and sodium iodide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
melting temperature of the apoprotein
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
amylose bead column chromatography, heparin column chromatography, and Superdex 200 gel filtration
His Trap column chromatography and Superdex 75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-(DE3)R3 Rosetta cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hewitt, S.N.; Dranow, D.M.; Horst, B.G.; Abendroth, J.A.; Forte, B.; Hallyburton, I.; Jansen, C.; Baragana, B.; Choi, R.; Rivas, K.L.; Hulverson, M.A.; Dumais, M.; Edwards, T.E.; Lorimer, D.D.; Fairlamb, A.H.; Gray, D.W.; Read, K.D.; Lehane, A.M.; Kirk, K.; Myler, P.J.; Wernimont, A.; Walpole, C.; Stacy, R.; Bar, B.a.r.r.
Biochemical and Structural characterization of selective allosteric inhibitors of the Plasmodium falciparum drug target, prolyl-tRNA-synthetase
ACS Infect. Dis.
3
34-44
2017
Plasmodium falciparum (Q8I5R7), Plasmodium falciparum
Manually annotated by BRENDA team
Jain, V.; Kikuchi, H.; Oshima, Y.; Sharma, A.; Yogavel, M.
Structural and functional analysis of the anti-malarial drug target prolyl-tRNA synthetase
J. Struct. Funct. Genomics
15
181-190
2014
Plasmodium falciparum (Q8I5R7), Plasmodium falciparum
Manually annotated by BRENDA team
Jain, V.; Yogavel, M.; Oshima, Y.; Kikuchi, H.; Touquet, B.; Hakimi, M.A.; Sharma, A.
Structure of prolyl-tRNA synthetase-halofuginone complex provides basis for development of drugs against malaria and toxoplasmosis
Structure
23
819-829
2015
Plasmodium falciparum (Q8I5R7), Toxoplasma gondii
Manually annotated by BRENDA team