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Information on EC 6.1.1.14 - glycine-tRNA ligase and Organism(s) Arabidopsis thaliana and UniProt Accession Q8L785

for references in articles please use BRENDA:EC6.1.1.14
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q8L785 not found.
Word Map
The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota
The taxonomic range for the selected organisms is: Arabidopsis thaliana
Synonyms
GARS, Glycine--tRNA ligase, Glycyl translase, glycyl tRNA synthetase, Glycyl-transfer ribonucleate synthetase, Glycyl-transfer ribonucleic acid synthetase, Glycyl-transfer RNA synthetase, Glycyl-tRNA synthetase, glycyl-tRNA synthetase 1, GlyRS, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glycine--tRNA ligase
Glycyl translase
Glycyl-transfer ribonucleate synthetase
Glycyl-transfer ribonucleic acid synthetase
Glycyl-transfer RNA synthetase
Glycyl-tRNA synthetase
GlyRS
GRS1
302
gene name
Synthetase, glycyl-transfer ribonucleate
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Aminoacylation
esterification
PATHWAY SOURCE
PATHWAYS
MetaCyc
SYSTEMATIC NAME
IUBMB Comments
glycine:tRNAGly ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-62-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + glycine + tRNAGly
AMP + diphosphate + glycyl-tRNAGly
show the reaction diagram
-
-
?
ATP + glycine + tRNAGly
AMP + diphosphate + glycyl-tRNAGly
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + glycine + tRNAGly
AMP + diphosphate + glycyl-tRNAGly
show the reaction diagram
O23627, Q8L785
-
-
?
ATP + glycine + tRNAGly
AMP + diphosphate + glycyl-tRNAGly
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in the early-globular stage
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
isozyme GlyRS1, active
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
SYGM2_ARATH
1067
0
119773
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE
117000
isozyme GlyRS2
27000
2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE
80000
isozyme GlyRS1
117000
x * 117000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 30000, about, partially processed isozyme GlyRS2, SDS-PAGE
?
x * 117000, SDS-PAGE
dimer or trimer
2-3 * 27000, about, partially processed isozyme GlyRS1, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
direction of a marker protein into isolated pea chloroplasts by the N-terminal domain of the enzyme, construction of the embryo-defective development mutant edd1 by usage of transposons as insertional mutagens, the reversible mutation leads to arrest of embryo growth and is lethal, generation of transgenic plants
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
recombinant isozyme GlyRS1 from Escherichia coli
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis of full-length edd1 wild-type gene, encoding the enzyme, gene contains a chloroplast targeting sequence, determination of the promoter, functional complementation of enzyme-deficient Arabidopsis plants thereby generation of transgenic plants
expressed in Saccharomyces cerevisiae grs1-deficient cells
-
overexpression of isozyme GlyRS1 in Escherichia coli strain TG2
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Duchene, A.M.; Peeters, N.; Dietrich, A.; Cosset, A.; Small, I.D.; Wintz, H.
Overlapping destinations for two dual targeted glycyl-tRNA synthetases in Arabidopsis thaliana and Phaseolus vulgaris
J. Biol. Chem.
276
15275-15283
2001
Arabidopsis thaliana, Arabidopsis thaliana (O23627), Arabidopsis thaliana (Q8L785), Phaseolus vulgaris
Manually annotated by BRENDA team
Uwer, U.; Willmitzer, L.; Altmann, T.
Inactivation of a glycyl-tRNA synthetase leads to an arrest in plant embryo development
Plant Cell
10
1277-1294
1998
Arabidopsis thaliana (Q8L785)
Manually annotated by BRENDA team
Chien, C.I.; Chen, Y.W.; Wu, Y.H.; Chang, C.Y.; Wang, T.L.; Wang, C.C.
Functional substitution of a eukaryotic glycyl-tRNA synthetase with an evolutionarily unrelated bacterial cognate enzyme
PLoS ONE
9
e94659
2014
Arabidopsis thaliana, Homo sapiens, Saccharomyces cerevisiae, Thermus thermophilus
Manually annotated by BRENDA team
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