Information on EC 6.1.1.13 - D-alanine-poly(phosphoribitol) ligase

for references in articles please use BRENDA:EC6.1.1.13
Word Map on EC 6.1.1.13
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.1.1.13
-
RECOMMENDED NAME
GeneOntology No.
D-alanine-poly(phosphoribitol) ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
show the reaction diagram
A thioester bond is formed transiently between D-alanine and the sulfhydryl group of the 4'-phosphopantetheine prosthetic group of D-alanyl carrier protein during the activation of the alanine. Involved in the synthesis of teichoic acids
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-Alanine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
D-alanine:poly(phosphoribitol) ligase (AMP-forming)
A thioester bond is formed transiently between D-alanine and the sulfhydryl group of the 4'-phosphopantetheine prosthetic group of D-alanyl carrier protein during the activation of the alanine. Involved in the synthesis of teichoic acids.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-65-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-2-amino-n-butyric acid + membrane fragments
D-2-Amino-n-butryl-membrane fragments + AMP + diphosphate
show the reaction diagram
-
not L-2-amino-n-butyric acid
-
-
ATP + D-Ala + D-alanyl carrier protein
D-Alanyl-D-alanyl carrier protein + AMP + diphosphate
show the reaction diagram
ATP + D-Ala + hydroxylamine
D-Alanine hydroxamate + AMP + diphosphate
show the reaction diagram
-
D-Ala can be substituted by D-2-amino-n-butyric acid with 14.6% and D-Ser with 7.5% efficiency
-
-
ATP + D-Ala + membrane fragments
D-Alanyl-membrane fragments + AMP + diphosphate
show the reaction diagram
-
ATP can be substituted by ADP, GTP, CTP, UTP, not by AMP, D-Ala cannot be substituted by L-Ala
-
-
ATP + D-alanine + D-alanyl lipoteichoic acid
AMP + diphosphate + D-alanyl lipoteichoic acid
show the reaction diagram
-
-
-
-
?
ATP + D-alanine + D-alanyl teichoic acid
AMP + diphosphate + D-alanyl teichoic acid
show the reaction diagram
ATP + D-alanine + poly(ribitol phosphate)
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
show the reaction diagram
ATP + diphosphate
ATP + diphosphate
show the reaction diagram
-
-
-
-
D-Alanine + ATP + ?
?
show the reaction diagram
-
biosynthesis of teichoic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-alanine + D-alanyl lipoteichoic acid
AMP + diphosphate + D-alanyl lipoteichoic acid
show the reaction diagram
-
-
-
-
?
ATP + D-alanine + D-alanyl teichoic acid
AMP + diphosphate + D-alanyl teichoic acid
show the reaction diagram
ATP + D-alanine + poly(ribitol phosphate)
AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
show the reaction diagram
D-Alanine + ATP + ?
?
show the reaction diagram
-
biosynthesis of teichoic acid
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
Ba2+ can replace Mg2+ in activity enhancement
Ca2+
-
Ca2+ can replace Mg2+ in activity enhancement
Co2+
-
Co2+ can replace Mg2+ in activity enhancement
K+
-
K+ can replace Mg2+ in activity enhancement
Mg2+
-
enhancement of activity, Mg2+ can be replaced by Ba2+, Ca2+, Co2+, K+, Na+
Na+
-
Na+ can replace Mg2+ in activity enhancement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-2-Amino-n-butyric acid hydroxamate
-
-
DL-2-Amino-n-butyric acid hydroxamate
-
-
DL-Alanine amide
-
-
DL-Alanine hydroxamate
-
-
additional information
-
not inhibitory are D-Ala, L-Ala, L-2-amino-n-butyric acid, Gly, D-Thr, D-Ile, L-Ile, D-cycloserine, L-cycloserine, DL-alaniol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DltD
-
facilitator of D-Ala ligation to the carrier protein Dcp
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5 - 3.3
ATP
0.85
D-2-amino-n-butyric acid
-
-
0.018 - 70
D-Ala
0.13
diphosphate
-
ATP-diphosphate exchange reaction
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
substrates D-Ala + hydroxylamine + ATP
7.5 - 8
-
ATP-diphosphate exchange reaction
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Bacillus subtilis (strain 168);
Q99ZA6
Streptococcus pyogenes serotype M1;
Q5XBN5
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
gel filtration
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, ammonium sulfate fraction stable for 6 months
-
-20°C, chromatographed fraction loses 25% of activity in 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (547 entries)
Please use the Sequence Search for a specific query.