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Information on EC 5.6.1.8 - myosin ATPase

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EC Tree
IUBMB Comments
Proteins of the contractile apparatus of muscle and nonmuscle cells; myosin molecule consists of two heavy chains (about 200 kDa) and two pairs of light chains (15--27 kDa). The head region of the heavy chain contains actin- and ATP-binding sites. ATP hydrolysis provides energy for actomyosin contraction.
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UNIPROT: P08799
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
myosin bound to actin filament at position n
=
+
+
myosin bound to actin filament at position n+1
Synonyms
myosin, actomyosin, heavy meromyosin, actomyosin atpase, nmiib, myosin-2, myosin-atpase, natural actomyosin, myosin 2, myosin ii atpase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
myosin 2 motor domain
-
actomyosin
-
-
-
-
matpase
-
-
-
-
myosin S1 ATPase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + myosin bound to actin filament at position n = ADP + phosphate + myosin bound to actin filament at position n+1
show the reaction diagram
combined quantum/classical mechanics study and catalytic mechanism, detailed overview and analysis
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphonic ester
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (actin-translocating)
Proteins of the contractile apparatus of muscle and nonmuscle cells; myosin molecule consists of two heavy chains (about 200 kDa) and two pairs of light chains (15--27 kDa). The head region of the heavy chain contains actin- and ATP-binding sites. ATP hydrolysis provides energy for actomyosin contraction.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + myosin bound to actin filament at position n
ADP + phosphate + myosin bound to actin filament at position n+1
show the reaction diagram
additional information
?
-
during its contraction cycle, the myosin motor catalyzes the hydrolysis of ATP
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + myosin bound to actin filament at position n
ADP + phosphate + myosin bound to actin filament at position n+1
show the reaction diagram
-
-
-
?
additional information
?
-
during its contraction cycle, the myosin motor catalyzes the hydrolysis of ATP
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required, Mg2+ coordination sphere, binding structure, overview
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole
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2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole
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2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
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2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
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2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
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2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol
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2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0919
2,3,4-tribromo-5-(3,5-dibromo-2-methoxyphenyl)-1H-pyrrole
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.2745
2,3,4-trichloro-5-(3,5-dichloro-2-methoxyphenyl)-1H-pyrrole
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.1254
2,4-dibromo-6-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.0244
2,4-dibromo-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.0472
2,4-dichloro-6-(3,4,5-tribromo-1H-pyrrol-2-yl)phenol
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.1263
2,4-dichloro-6-(3,4,5-trichloro-1H-pyrrol-2-yl)phenol
Dictyostelium discoideum
pH not specified in the publication, 25°C
0.1822
2-(3,4,5-tribromo-1-methyl-1H-pyrrol-2-yl)phenol
Dictyostelium discoideum
pH not specified in the publication, 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
myosin is a linear motor that utilizes the free energy obtained from ATP hydrolysis to drive the coordinated motion of its protein domains during muscle contraction. During its contraction cycle, the myosin motor catalyzes the hydrolysis of ATP, molecular mechanism involving residue Glu459, overview
additional information
combined quantum/classical mechanics study and catalytic mechanism overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MYS2_DICDI
2116
0
243786
Swiss-Prot
other Location (Reliability: 5)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis, in which myosin II from Dictyostelium discoideum is complexed with Mg2-ADP-vanadate, a transition state analogue, PDB ID 1VOM
hanging drop vapor diffusion method
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged motor domain construct comprising amino acids 1-761
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Preller, M.; Chinthalapudi, K.; Martin, R.; Knolker, H.J.; Manstein, D.J.
Inhibition of myosin ATPase activity by halogenated pseudilins: a structure-activity study
J. Med. Chem.
54
3675-3685
2011
Dictyostelium discoideum (P08799)
Manually annotated by BRENDA team
Kiani, F.A.; Fischer, S.
Advances in quantum simulations of ATPase catalysis in the myosin motor
Curr. Opin. Struct. Biol.
31
115-123
2015
Dictyostelium discoideum (P08799)
Manually annotated by BRENDA team