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Information on EC 5.5.1.B7 - clerodienyl diphosphate synthase and Organism(s) Salvia divinorum and UniProt Accession A0A1S5RW73

for references in articles please use BRENDA:EC5.5.1.B7
preliminary BRENDA-supplied EC number
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Salvia divinorum
UNIPROT: A0A1S5RW73 not found.
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The taxonomic range for the selected organisms is: Salvia divinorum
The expected taxonomic range for this enzyme is: Salvia divinorum
Synonyms
clpp synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
clerodienyl diphosphate synthase
A0A1L3THX5
-
CLPP synthase
A0A1L3THX5
-
CPS2
A0A1L3THX5
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl diphosphate-lyase (cyclizing, (-)-kolavenol-forming)
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
(-)-kolavenol
show the reaction diagram
A0A1L3THX5
-
-
-
?
geranylgeranyl diphosphate
clerodienyl diphosphate + H+
show the reaction diagram
A0A1L3THX5
the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate
-
-
?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
A0A1L3THX5
specific expression in trichomes
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
A0A1L3THX5
the enzyme catalyzes the committed step in salvinorin A biosynthesis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F255A/N313A
A0A1L3THX5
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol
F255H
A0A1L3THX5
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-affinity column chromatography and Superdex 200 gel filtration
A0A1L3THX5
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells
A0A1L3THX5
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pelot, K.A.; Mitchell, R.; Kwon, M.; Hagelthorn, D.M.; Wardman, J.F.; Chiang, A.; Bohlmann, J.; Ro, D.K.; Zerbe, P.
Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase
Plant J.
89
885-897
2017
Salvia divinorum (A0A1L3THX5), Salvia divinorum
Manually annotated by BRENDA team