Any feedback?
Information on EC 5.5.1.4 - inositol-3-phosphate synthase and Organism(s) Oryza sativa and UniProt Accession O64437
for references in articles please use BRENDA:EC5.5.1.4Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
5.5.1.4 inositol-3-phosphate synthaseIUBMB Comments
Requires NAD+, which dehydrogenates the -CHOH- group to -CO- at C-5 of the glucose 6-phosphate, making C-6 into an active methylene, able to condense with the -CHO at C-1. Finally, the enzyme-bound NADH reconverts C-5 into the -CHOH- form.
Specify your search results
Word Map
- 5.5.1.4
-
nucleation
-
neuroblast
-
phytic
-
ice-nucleating
-
monophosphatase
-
cloud
-
halophytic
-
inositol-containing
-
cyclitols
- phytate
-
supercooled
- galactinol
-
hexakisphosphate
-
radiative
-
2-kinase
- impase
- medicine
- pharmacology
- biotechnology
- synthesis
The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
inps, myo-inositol-1-phosphate synthase, mips1, inositol-1-phosphate synthase, mip synthase, pip synthase, myo-inositol 1-phosphate synthase, inositol-3-phosphate synthase, l-myo-inositol-1-phosphate synthase, sll1981, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1L-myo-Inositol-1-phosphate synthase
-
-
-
-
D-Glucose 6-phosphate cycloaldolase
-
-
-
-
D-Glucose 6-phosphate-1L-myoinositol 1-phosphate cyclase
-
-
-
-
D-Glucose 6-phosphate-1L-myoinositol 1-phosphate cycloaldolase
-
-
-
-
D-Glucose 6-phosphate-L-myo-inositol 1-phosphate cyclase
-
-
-
-
D-Glucose-6-phosphate,L-myo-inositol-1-phosphate cycloaldolase
-
-
-
-
Glucocycloaldolase
-
-
-
-
Glucose 6-phosphate cyclase
-
-
-
-
Glucose-6-phosphate inositol monophosphate cycloaldolase
-
-
-
-
Inositol 1-phosphate synthase
-
-
-
-
Inositol 1-phosphate synthetase
-
-
-
-
INPS1
-
-
-
-
IPS
-
-
-
-
L-myo-Inositol 1-phosphate synthetase
-
-
-
-
L-myo-Inositol-1-phosphate synthase
-
-
-
-
MI-1-P synthase
-
-
-
-
MIP synthase
-
-
-
-
MIPS
myo-Inositol-1-P synthase
-
-
-
-
Myo-inositol-1-phosphate synthase
-
-
-
-
Synthase, myo-inositol 1-phosphate
-
-
-
-
MIPS
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cyclization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-3-phosphate lyase (isomerizing)
Requires NAD+, which dehydrogenates the -CHOH- group to -CO- at C-5 of the glucose 6-phosphate, making C-6 into an active methylene, able to condense with the -CHO at C-1. Finally, the enzyme-bound NADH reconverts C-5 into the -CHOH- form.
CAS REGISTRY NUMBER
COMMENTARY
9032-95-5
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-Glucose 6-phosphate
L-myo-Inositol 1-phosphate
-
absolute specificity for
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
Km value of wild-type 0.188 mM, Km value of mutant bearing amino acids 174-210 of Porteresia coarctata enzyme 0.451 mM
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.19
D-glucose 6-phosphate
mutant bearing amino acids 174-210 of Porteresia coarctata enzyme, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
8 - 8.5
mutant bearing amino acids 174-210 of Porteresia coarctata enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
isoenzyme OsMIPS1-2: low expression expression comparable to splice varian OsMIPS1
isoenzyme OsMIPS1-3: OsMIPS1-3 shows a more or less constitutive expression in different abiotic stresses in shoots, roots, panicle stages, and various developing seed stages
isoenzyme OsMIPS1-3: OsMIPS1-3 shows a more or less constitutive expression in different abiotic stresses in shoots, roots, panicle stages, and various developing seed stages
isoenzyme OsMIPS1-1: expression is largely responsive to abiotic stress in the root tissue, its expression is induced by drought, NaCl, cold, abscisic acid, brassinosteroid and salicylic acid
isoenzyme OsMIPS1-2: low expression expression comparable to splice varian OsMIPS1
isoenzyme OsMIPS1-1: expression during seed development comparable to splice variant OsMIPS1: Seed of 20 DAP stage has the highest expression followed by 10 and 30 DAP stages
isoenzyme OsMIPS1-2: expression during seed development comparable to splice variant OsMIPS1: Seed of 20 DAP stage has the highest expression followed by 10 and 30 DAP stages
isoenzyme OsMIPS1-3: OsMIPS1-3 shows a more or less constitutive expression in different abiotic stresses in shoots, roots, panicle stages, and various developing seed stages
isoenzyme OsMIPS1-3: OsMIPS1-3: expression during seed development comparable to splice variant OsMIPS1: Seed of 20 DAP stage has the highest expression followed by 10 and 30 DAP stages
isoenzyme OsMIPS1: expression largely seed-specific: Seed of 20 DAP stage has the highest expression followed by 10 and 30 DAP stages
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
seed-specific silencing of MIPS in transgenic rice plants leads to substantial reduction in levels of phytic acid along with an increase in phosphate content. Low phytate seeds have an undesirable diminution in levels of myo-inositol and ascorbate possibly leading to sensitiveness of seeds to abscisic acid during germination
physiological function
physiological function
-
MIPS is a pivotal biosynthetic enzyme in the myo-inositol pathway, including the synthesis of phytic acid
physiological function
-
expression of myo-inositol 3-phosphate synthase confers salt tolerance in rice
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60000
59000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
a 37-amino acid stretch between W174 and S210 is the salt-tolerance determinant domain of Porteresia coarctata enzyme. Introduction of this stretch into Oryza sativa enzyme provides salt tolerance properties
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
isoenzyme OsMIPS1-1: expression is largely responsive to abiotic stress in the root tissue, its expression is induced by drought, NaCl, cold, ABA, BR and SA
isoenzyme OsMIPS2: expression is considerably high in the unfertilized flower and during panicle development
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
RayChaudhuri, A.; Hait, N.C.; das Gupta, S.; Bhaduri, T.J.; Deb, R.; Majumder, A.L.
L-myo-Inositol 1-phosphate synthase from plant sources
Plant Physiol.
115
727-736
1997
Saccharomyces cerevisiae, Euglena gracilis, Oryza sativa, Vigna radiata, Arthrospira platensis
Majee, M.; Maitra, S.; Ghosh Dastidar, K.; Pattnaik, S.; Chatterjee, A.; Hait, N.C.; Das, K.P.; Majumder, A.L.
A novel salt-tolerant L-myo-inositol 1-phosphate synthase from Porteresia coarctata (Roxb.)Tateoka, a halophytic wild rice Molecular cloning, bacterial overexpression, characterization and functional introgression into tobacco conferring salt-tolerance phenotype
J. Biol. Chem.
279
28539-28552
2004
Oryza sativa (O64437), Oryza sativa, Oryza coarctata (Q7XZE6), Oryza coarctata
Ghosh Dastidar, K.; Maitra, S.; Goswami, L.; Roy, D.; Das, K.P.; Majumder, A.L.
An insight into the molecular basis of salt tolerance of L-myo-inositol 1-P synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice
Plant Physiol.
140
1279-1296
2006
Oryza sativa (O64437), Oryza coarctata (Q7XZE6), Oryza coarctata
Kuwano, M.; Mimura, T.; Takaiwa, F.; Yoshida, K.T.
Generation of stable low phytic acid transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene (RINO1) using the 18-kDa oleosin promoter
Plant Biotechnol. J.
7
96-105
2009
Oryza sativa
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana, Archaeoglobus fulgidus, Brassica napus, Citrus x paradisi, Euglena gracilis, Glycine max, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Perilla frutescens, Vigna radiata, Phaseolus vulgaris, Pisum sativum, Sesamum indicum, Solanum tuberosum, Zea mays
Ray, S.; Patra, B.; Das-Chatterjee, A.; Ganguli, A.; Majumder, A.L.
Identification and organization of chloroplastic and cytosolic L-myo-inositol 1-phosphate synthase coding gene(s) in Oryza sativa: comparison with the wild halophytic rice, Porteresia coarctata
Planta
231
1211-1227
2010
Oryza coarctata (B6V6K8), Oryza coarctata (B6V6K9), Oryza coarctata, Oryza sativa (O64437), Oryza sativa
Khurana, N.; Chauhan, H.; Khurana, P.
Expression analysis of a heat-inducible, myo-inositol-1-phosphate synthase (MIPS) gene from wheat and the alternatively spliced variants of rice and Arabidopsis
Plant Cell Rep.
31
237-251
2012
Arabidopsis thaliana, Arabidopsis thaliana (Q38862), Arabidopsis thaliana (Q9LX12), Oryza sativa, Oryza sativa (O64437), Triticum aestivum (G8HMZ0), Triticum aestivum (Q9S7U0), Triticum aestivum
Ali, N.; Paul, S.; Gayen, D.; Sarkar, S.N.; Datta, S.K.; Datta, K.
RNAi mediated down regulation of myo-inositol-3-phosphate synthase to generate low phytate rice
Rice (N.Y.)
6
12
2013
Oryza sativa
Kusuda, H.; Koga, W.; Kusano, M.; Oikawa, A.; Saito, K.; Hirai, M.Y.; Yoshida, K.T.
Ectopic expression of myo-inositol 3-phosphate synthase induces a wide range of metabolic changes and confers salt tolerance in rice
Plant Sci.
232
49-56
2015
Oryza sativa
Basak, P.; Maitra-Majee, S.; Das, J.K.; Mukherjee, A.; Ghosh Dastidar, S.; Pal Choudhury, P.; Lahiri Majumder, A.
An evolutionary analysis identifies a conserved pentapeptide stretch containing the two essential lysine residues for rice L-myo-inositol 1-phosphate synthase catalytic activity
PLoS ONE
12
e0185351
2017
Oryza sativa (Q8S5N2), Oryza sativa
EXTERNAL LINKS (specific for EC-Number 5.5.1.4)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
