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EC Tree
IUBMB Comments The enzyme, characterized from the plant Nepeta mussinii, binds an NAD+ cofactor. The product is a precursor of (+)-cis,cis-nepetalactone, one of the stereoisomers responsible for the psychoactive effects catnip has on cats.
The expected taxonomic range for this enzyme is: Nepeta racemosa
Synonyms
nepetalactol-related SDR 3,
NEPS3 ,
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nepetalactol-related SDR 3
A0A4P1LYE8
-
NEPS3
-
-
-
-
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(S)-8-oxocitronellyl enol = (+)-cis,cis-nepetalactol
-
-
-
-
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(S)-8-oxocitronellyl enol cyclase [(+)-cis,cis-nepetalactol-forming]
The enzyme, characterized from the plant Nepeta mussinii, binds an NAD+ cofactor. The product is a precursor of (+)-cis,cis-nepetalactone, one of the stereoisomers responsible for the psychoactive effects catnip has on cats.
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(S)-8-oxocitronellyl enol
(+)-cis,cis-nepetalactol
A0A4P1LYE8
-
-
-
?
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(S)-8-oxocitronellyl enol
(+)-cis,cis-nepetalactol
A0A4P1LYE8
-
-
-
?
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NAD+
A0A4P1LYE8
enzyme binds NAD+. NEPS3 shows no notable oxidation activity with NAD+ or NADP+ or at different pHs
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-
A0A4P1LYE8
UniProt
brenda
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A0A4P1LYE8
glandular trichome
brenda
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physiological function
A0A4P1LYE8
isoforms Neps1 and Neps2 catalyze the stereoselective cyclisation of reactive intermediate (S)-8-oxocitronellyl enol into nepetalactol. Subsequent oxidation of nepetalactol by NEPS1 provides nepetalactone, metabolites known for both insect-repellent activity and euphoric effect in cats
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NEPS3_NEPRA
270
0
28179
Swiss-Prot
other Location (Reliability: 3 )
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in complex with NAD+. Cofactor is not utilized during catalysis, but appears to be important for the enzyme structure
A0A4P1LYE8
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A151T
A0A4P1LYE8
mutation abolishes detectable soluble expression
G152T
A0A4P1LYE8
mutation abolishes detectable soluble expression
K169M
A0A4P1LYE8
severe reduction of activity
L156S
A0A4P1LYE8
reduced dehydrogenase activity
M196S
A0A4P1LYE8
severe reduction of activity
N150T
A0A4P1LYE8
activity similar to wild-type
S153P
A0A4P1LYE8
mutation abolishes detectable soluble expression
S154L
A0A4P1LYE8
no detectable cis-cis cyclase activity, though at higher enzyme concentrations formation of cis,trans-nepetalactol, reaction of EC 5.5.1.34
Y165F
A0A4P1LYE8
mutation abolishes detectable soluble expression
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Lichman, B.R.; Kamileen, M.O.; Titchiner, G.R.; Saalbach, G.; Stevenson, C.E.M.; Lawson, D.M.; O'Connor, S.E.
Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis
Nat. Chem. Biol.
15
71-79
2019
Nepeta racemosa (A0A4P1LYE8)
brenda
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History
Enzyme Nomenclature
5.5.1.35 (+)-cis,cis-nepetalactol synthase
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