Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 5.5.1.17 - (S)-beta-macrocarpene synthase and Organism(s) Zea mays and UniProt Accession Q5GJ60

for references in articles please use BRENDA:EC5.5.1.17
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
The synthesis of (S)-beta-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-beta-bisabolene (cf. EC 4.2.3.55, (S)-beta-bisabolene synthase). The second step is the isomerization to (S)-beta-macrocarpene.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Zea mays
UNIPROT: Q5GJ60
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Zea mays
The expected taxonomic range for this enzyme is: Zea mays
Synonyms
terpene synthase 11, terpene synthase 6, TPS11, TPS6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
terpene synthase 6
-
terpene synthase 11
-
TPS11
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-beta-macrocarpene lyase (decyclizing)
The synthesis of (S)-beta-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-beta-bisabolene (cf. EC 4.2.3.55, (S)-beta-bisabolene synthase). The second step is the isomerization to (S)-beta-macrocarpene.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-beta-bisabolene
(S)-beta-macrocarpene
show the reaction diagram
geranyl diphosphate
(S)-beta-bisabolene + (S)-beta-macrocarpene
show the reaction diagram
-
in the presence of geranylgeranyl diphosphate, TPS11 also catalyzes the formation of beta-myrcene and linalool, along with minor amounts of limonene, alpha-thujene, sabinene, and alpha-terpinolene
-
?
(S)-beta-bisabolene
(S)-beta-macrocarpene
show the reaction diagram
geranyl diphosphate
(S)-beta-bisabolene + (S)-beta-macrocarpene
show the reaction diagram
-
in the presence of geranylgeranyl diphosphate, TPS11 also catalyzes the formation of beta-myrcene and linalool, along with minor amounts of limonene, alpha-thujene, sabinene, and alpha-terpinolene
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-beta-bisabolene
(S)-beta-macrocarpene
show the reaction diagram
(S)-beta-bisabolene
(S)-beta-macrocarpene
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
the enzyme is mostly stimulated by 10 mM Mg2+
Mn2+
the enzyme is stimulated by 0.25 mM Mn2+. In the presence of Mn2+, there is increased formation of (S)-beta-bisabolene
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0011
geranyl diphosphate
recombinant enzyme, in the presence of 0.05 mM Mn2+, pH and temperature not specified in the publication
0.0011
geranyl diphosphate
recombinant enzyme, in the presence of 0.05 mM Mn2+, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
forming beta-bisabolene
7
forming beta-macrocarpene
8.5
forming beta-bisabolene
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8.6
half-maximal activity at pH 6.2 and at pH 8.6, in presence of 5 mM Mg2+. Within a pH range from 5.0 to 8.0, the major product is (S)-beta-macrocarpene, but higher pH values favor the formation of (S)-beta-bisabolene
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme may be involved in plant defense
physiological function
the enzyme may be involved in plant defense
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPS6_MAIZE
548
0
63731
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D526N
inactive
Y522F
the mutation strongly reduces the rate of reprotonation of (S)-beta-bisabolene
D526N
Y522F
the mutation strongly reduces the rate of reprotonation of (S)-beta-bisabolene
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial purification
partial purification
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Top10 cells
overexpression in Escherichia coli
expressed in Escherichia coli Top10 cells
overexpression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after leaf damage by Spodoptera littoralis, the transcript level of TSP6 is elevated
after leaf damage by Spodoptera littoralis, the transcript level of TSP11 is elevated
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kllner, T.G.; Schnee, C.; Li, S.; Svatos, A.; Schneider, B.; Gershenzon, J.; Degenhardt, J.
Protonation of a neutral (S)-beta-bisabolene intermediate is involved in (S)-beta-macrocarpene formation by the maize sesquiterpene synthases TPS6 and TPS11
J. Biol. Chem.
283
20779-20788
2008
Zea mays (Q1EG72), Zea mays (Q5GJ60)
Manually annotated by BRENDA team
Huffaker, A.; Kaplan, F.; Vaughan, M.M.; Dafoe, N.J.; Ni, X.; Rocca, J.R.; Alborn, H.T.; Teal, P.E.; Schmelz, E.A.
Novel acidic sesquiterpenoids constitute a dominant class of pathogen-induced phytoalexins in maize
Plant Physiol.
156
2082-2097
2011
Zea mays (Q1EG72), Zea mays (Q5GJ60)
Manually annotated by BRENDA team