Information on EC 5.5.1.16 - halimadienyl-diphosphate synthase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
5.5.1.16
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RECOMMENDED NAME
GeneOntology No.
halimadienyl-diphosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate = tuberculosinyl diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tuberculosinol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
halima-5,13-dien-15-yl-diphosphate lyase (decyclizing)
Requires Mg2+ for activity. This enzyme is found in pathogenic prokaryotes such as Mycobacterium tuberculosis but not in non-pathogens such as Mycobacterium smegmatis so may play a role in pathogenicity. The product of the reaction is subsequently dephosphorylated yielding tuberculosinol (halima-5,13-dien-15-ol).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E,E,E)-geranylgeranyl diphosphate
halimadienyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate
halima-5(6),13-dien-15-yl diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E,E,E)-geranylgeranyl diphosphate
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substrate inhibition
15-aza-14,15-dihydrogeranylgeranyl diphosphate
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15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
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Mg2+
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the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
(E,E,E)-geranylgeranyl diphosphate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
(E,E,E)-geranylgeranyl diphosphate
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018
(E,E,E)-geranylgeranyl diphosphate
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000002
15-aza-14,15-dihydrogeranylgeranyl diphosphate
Mycobacterium tuberculosis
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0.0000001
15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
Mycobacterium tuberculosis
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-4
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freezing leads to loss of about 10% activity over the course of 1 week
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, less than 24 h, the enzyme retains essentially full activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amylose resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain C41
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expression in Escherichia coli
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