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Information on EC 5.5.1.15 - terpentedienyl-diphosphate synthase for references in articles please use BRENDA:EC5.5.1.15Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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terpentedienyl-diphosphate synthase
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geranylgeranyl diphosphate = terpentedienyl diphosphate
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terpentecin biosynthesis
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Biosynthesis of antibiotics
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terpentedienyl-diphosphate lyase (decyclizing)
Requires Mg2+. Contains a DXDD motif, which is a characteristic of diterpene cylases whose reactions are initiated by protonation at the 14,15-double bond of geranylgeranyl diphosphate (GGDP) [2]. The triggering proton is lost at the end of the cyclization reaction [3]. The product of the reaction, terpentedienyl diphosphate, is the substrate for EC 4.2.3.36, terpentetriene synthase and is a precursor of the diterpenoid antibiotic terpentecin.
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geranylgeranyl diphosphate
terpentedienyl diphosphate
geranylgeranyl diphosphate
terpentedienyl diphosphate
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geranylgeranyl diphosphate
terpentedienyl diphosphate
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geranylgeranyl diphosphate
terpentedienyl diphosphate
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the triggering proton is lost at the end of the cyclization reaction
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Mg2+
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the enzyme activity of the Cyc1 is highest at a concentration of 1 mM but slightly inhibited at a concentration of 10 mM (decreased 40%)
additional information
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no activity is detected with other divalent metal ions such as Ca2+, Co2+, Cu2+, Fe2+, Mn2+, and Zn2+ at both 1 and 10 mM
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geranylgeranyl diphosphate
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above 0.05 mM
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additional information
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20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cyc1
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0.0642
geranylgeranyl diphosphate
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25 - 50
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25-30: optimum, 50°C, no activity detected above
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30
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full activity after incubation at 30°C in 0.05 M Tris-HCl buffer for 1 h
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heterologous expression of the cyclase genes in Streptomyces lividans and Escherichia coli
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CYC1_KITGR
499
54163
Swiss-Prot
A0A2P4UKC2_9ACTN
529
55644
TrEMBL
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Dairi, T.; Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.
Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin
J. Bacteriol.
183
6085-6094
2001
Kitasatospora griseola, Kitasatospora griseola MF730-N6
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Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T.
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin
J. Biol. Chem.
277
37098-37104
2002
Kitasatospora griseola
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Eguchi, T.; Dekishima, Y.; Hamano, Y.; Dairi, T.; Seto, H.; Kakinuma, K.
A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase
J. Org. Chem.
68
5433-5438
2003
Streptomyces lividans
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