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IUBMB Comments Requires Mg2+ EC 4.2.3.36 , terpentetriene synthase and is a precursor of the diterpenoid antibiotic terpentecin.
Synonyms
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CPS:H315N/T316V
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double mutant H315N/T316V of ent-copalyl diphosphate synthase
terpentedienyl diphosphate synthase
A0A2M9LDX2
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TPP synthase
A0A2M9LDX2
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geranylgeranyl diphosphate = terpentedienyl diphosphate
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MetaCyc
terpentecin biosynthesis
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terpentedienyl-diphosphate lyase (decyclizing)
Requires Mg2+. Contains a DXDD motif, which is a characteristic of diterpene cylases whose reactions are initiated by protonation at the 14,15-double bond of geranylgeranyl diphosphate (GGDP) [2]. The triggering proton is lost at the end of the cyclization reaction [3]. The product of the reaction, terpentedienyl diphosphate, is the substrate for EC 4.2.3.36, terpentetriene synthase and is a precursor of the diterpenoid antibiotic terpentecin.
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geranylgeranyl diphosphate
terpentedienyl diphosphate
geranylgeranyl diphosphate
terpentedienyl diphosphate
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Substrates: -
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geranylgeranyl diphosphate
terpentedienyl diphosphate
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Substrates: -
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geranylgeranyl diphosphate
terpentedienyl diphosphate
A0A2M9LDX2
Substrates: -
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geranylgeranyl diphosphate
terpentedienyl diphosphate
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Substrates: -
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geranylgeranyl diphosphate
terpentedienyl diphosphate
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Substrates: the triggering proton is lost at the end of the cyclization reaction
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geranylgeranyl diphosphate
terpentedienyl diphosphate
A0A2M9LDX2
Substrates: -
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additional information
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no activity is detected with other divalent metal ions such as Ca2+, Co2+, Cu2+, Fe2+, Mn2+, and Zn2+ at both 1 and 10 mM
Mg2+
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the enzyme activity of the Cyc1 is highest at a concentration of 1 mM but slightly inhibited at a concentration of 10 mM (decreased 40%)
Mg2+
A0A2M9LDX2
1 mM used in assay conditions
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geranylgeranyl diphosphate
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above 0.05 mM
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additional information
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20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cyc1
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0.0642
geranylgeranyl diphosphate
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25 - 50
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25-30: optimum, 50°C, no activity detected above
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A0A2M9LDX2
UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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CYC1_KITGR
499
0
54163
Swiss-Prot
Mitochondrion (Reliability: 5 )
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hanging drop vapor diffusion method, using 10 mM zinc sulfate heptahydrate, 25% (v/v) polyethylene glycol monomethyl ether 550, and 100 mM MES monohydrate, pH 6.5
A0A2M9LDX2
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D296A
A0A2M9LDX2
inactive
G485D
A0A2M9LDX2
the mutant converts geranylgeranyl diphosphate preferentially into syn-copalyl diphosphate
H342A
A0A2M9LDX2
inactive
H342F
A0A2M9LDX2
inactive
K383A
A0A2M9LDX2
inactive
Y489A
A0A2M9LDX2
inactive
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30
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full activity after incubation at 30°C in 0.05 M Tris-HCl buffer for 1 h
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HisTrap column chromatography and Superdex gel filtration
A0A2M9LDX2
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expressed in Escherichia coli NiCo cells (NEB) cells
A0A2M9LDX2
heterologous expression of the cyclase genes in Streptomyces lividans and Escherichia coli
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Dairi, T.; Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.
Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin
J. Bacteriol.
183
6085-6094
2001
Kitasatospora griseola, Kitasatospora griseola MF730-N6
brenda
Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T.
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin
J. Biol. Chem.
277
37098-37104
2002
Kitasatospora griseola
brenda
Eguchi, T.; Dekishima, Y.; Hamano, Y.; Dairi, T.; Seto, H.; Kakinuma, K.
A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase
J. Org. Chem.
68
5433-5438
2003
Streptomyces lividans
brenda
Schulte, S.; Potter, K.C.; Lemke, C.; Peters, R.J.
Catalytic bases and stereocontrol in Lamiaceae class II diterpene cyclases
Biochemistry
57
3473-3479
2018
Salvia miltiorrhiza
brenda
Stowell, E.; Ehrenberger, M.; Lin, Y.; Chang, C.; Rudolf, J.
Structure-guided product determination of the bacterial type II diterpene synthase Tpn2
Commun. Chem.
5
146
2022
Kitasatospora sp. CB02891 (A0A2M9LDX2)
brenda
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