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Information on EC 5.5.1.13 - ent-copalyl diphosphate synthase and Organism(s) Oryza sativa and UniProt Accession Q5MQ85

for references in articles please use BRENDA:EC5.5.1.13
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EC Tree
IUBMB Comments
Part of a bifunctional enzyme involved in the biosynthesis of kaurene. See also EC 4.2.3.19 (ent-kaurene synthase)
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This record set is specific for:
Oryza sativa
UNIPROT: Q5MQ85
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Word Map
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  • 5.5.1.13
  • gibberellin
  • diterpene
  • diterpenoids
  • geranylgeranyl
  • phytohormone
  • phytoalexins
  • ent-cpp
  • ent-kaurenoic
  • phytocassanes
  • e,e,e-geranylgeranyl
  • oscps4
  • 3-oxidase
  • ga-deficient
  • labdane-related
  • ga1
  • ditpss
  • oryzalexins
  • ga20ox
  • syn-copalyl
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ent-copalyl diphosphate synthase, oscps1, ent-cps, ent-kaurene synthase a, oscyc2, gfcps/ks, oscps2ent, oscps1ent, ent-kaurene synthetase a, copalyl synthase/kaurene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
OsCPS2ent
-
CPS1
-
isozyme
CPS2/Cyc2
-
bifunctional enzyme, acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins)
ent-kaurene synthase A
-
-
-
-
ent-kaurene synthetase A
-
-
-
-
OsCPS1
-
-
OsCPS1ent
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate lyase (decyclizing)
Part of a bifunctional enzyme involved in the biosynthesis of kaurene. See also EC 4.2.3.19 (ent-kaurene synthase)
CAS REGISTRY NUMBER
COMMENTARY hide
9055-64-5
in Chemical Abstracts not distinguished from EC 4.2.3.19
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
ent-copalyl diphosphate
show the reaction diagram
OsCPS2ent is involved in secondary metabolism producing defensive phytochemicals. OsCPS2ent mRNA is specifically induced in leaves prior to production of the corresponding phytoalexins. The transcriptional control of OsCPS2ent seems to be an important means of regulating defensive phytochemical biosynthesis
-
-
?
copalyl diphosphate
ent-kaurene + diphosphate
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate
ent-copalyl diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
ent-copalyl diphosphate
show the reaction diagram
OsCPS2ent is involved in secondary metabolism producing defensive phytochemicals. OsCPS2ent mRNA is specifically induced in leaves prior to production of the corresponding phytoalexins. The transcriptional control of OsCPS2ent seems to be an important means of regulating defensive phytochemical biosynthesis
-
-
?
geranylgeranyl diphosphate
ent-copalyl diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
the enzyme activity is highest at 0.1mM of Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-isopropyl-N,N,N,2-tetramethyl-4-[(1-piperidinylcarbonyl)oxy]anilinium chloride
-
CPS1 activity exhibits approximately 70% inhibition by 0.1 mM Amo-1618, whereas the OsCPS2/OsCyc2 activity exhibits approximately 10% inhibition
Co2+
-
35-60% relative activity in the presence of 0.1 mM Co2+ compared to Mg2+
Fe2+
-
5-10% relative activity in the presence of 0.1 mM Fe+ compared to Mg2+
geranylgeranyl diphosphate
-
CPS1 but not CPS2/Cyc2 is inhibited by 0.05-0.06 mM geranylgeranyl diphosphate, CPS1 shows significant substrate inhibition in the presence of more than 0.05 mM geranylgeranyl diphosphate
Mg2+
-
weak inhibition of CPS2/Cyc2 at 5mM Mg2+
Ni2+
-
60-70% relative activity in the presence of 0.1 mM Ni2+ compared to Mg2+
Zn2+
-
CPS1 shows 30% enzymatic activity with 0.1 mM Zn2+ compared to Mg2+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 40
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
OsCPS2ent
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
OsCPS2ent mRNA is specifically induced in leaves prior to production of the corresponding phytoalexins
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
TPS genes in both gymnosperms and angiosperms are likely derived from a duplication of an ancestral gene encoding a bifunctional kaurene synthase, TPS family size and comparison of physiological functions of TPS enzymes in different organisms, overview. The genomes of some model angiosperms and gymnosperms contain 40-152 TPS genes, not all of them functional and most of the functional ones having lost activity in either the CPS- or KS-type domains. TPS genes are generally divided into seven clades, with some plant lineages having a majority of their TPS genes in one or two clades, indicating lineage-specific expansion of specific types of genes. Evolutionary plasticity is evident in the TPS family, with closely related enzymes differing in their product profiles, subcellular localization, or the in planta substrates they use. Based on the reaction mechanism and products formed, plant TPSs can be classified into two groups: class I and class II, detailed overview
physiological function
-
the TPS gene encodes a copalyl synthase/kaurene synthase, CPS/KS, a bifunctional enzyme. Copalyl diphosphate synthase, CPS, and kaurene synthase, KS, convert geranylgeranyl diphosphate first to copalyl diphosphate, then to ent-kaurene, the precursor of all plant gibberellins
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80900
-
CPS2/Cyc2, calculated from amino acid sequence
84900
-
CPS1, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
activity is fully retained after incubating at 30°C in 0.05 M Tris-HCl at pH 7.0 for 1 h
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
-
5 mM 2-mercaptoethanol is required for full enzymatic activity
Glycerol
-
20% (v/v) glycerol is required for full enzymatic activity
Tween 80
-
0.1% (v/v) Tween 80 is required for full enzymatic activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
OsCPS2ent, expression in Escherichia coli BL21
expressed in Escherichia coli
-
gene TPS, genetic organization on the chromosome, genotyping and phylogenetic analysis, detailed overview
-
OsCPS1ent, expression in Escherichia coli BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Otomo, K.; Kenmoku, H.; Oikawa, H.; Konig, W.A.; Toshima, H.; Mitsuhashi, W.; Yamane, H.; Sassa, T.; Toyomasu, T.
Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis
Plant J.
39
886-893
2004
Oryza sativa, Oryza sativa (Q6Z5I0)
Manually annotated by BRENDA team
Prisic, S.; Xu, M.; Wilderman, P.R.; Peters, R.J.
Rice contains two disparate ent-copalyl diphosphate synthases with distinct metabolic functions
Plant Physiol.
136
4228-4236
2004
Oryza sativa, Oryza sativa (Q5MQ85)
Manually annotated by BRENDA team
Hayashi, Y.; Toyomasu, T.; Hirose, Y.; Onodera, Y.; Mitsuhashi, W.; Yamane, H.; Sassa, T.; Dairi, T.
Comparison of the enzymatic properties of ent-copalyl diphosphate-synthases in the biosynthesis of phytoalexins and gibberellins in rice
Biosci. Biotechnol. Biochem.
72
523-530
2008
Oryza sativa
Manually annotated by BRENDA team
Chen, F.; Tholl, D.; Bohlmann, J.; Pichersky, E.
The family of terpene synthases in plants: A mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom
Plant J.
66
212-229
2011
Abies grandis, Arabidopsis thaliana, Oryza sativa, Physcomitrium patens, Picea abies, Picea glauca, Populus trichocarpa, Sorghum bicolor, Vitis vinifera, Picea sitchensis, Selaginella moellendorffii, Picea engelmannii x Picea glauca
Manually annotated by BRENDA team