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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
alpha-pinene oxide lyase, lyase, alpha-pinene oxide, Pralpha-POL,
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lyase, alpha-pinene oxide
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alpha-pinene oxide lyase
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alpha-pinene oxide lyase
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alpha-pinene oxide lyase
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alpha-pinene oxide lyase
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Pralpha-POL
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alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
mechanism
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alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
pathway
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alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
pathway
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alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
mechanism
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alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
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intramolecular lyase reaction
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alpha-pinene-oxide lyase (decyclizing)
Both rings of pinene are cleaved in the reaction.
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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enzyme is involved in the pathway of the metabolism of alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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enzyme is involved in the pathway of the metabolism of alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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i.e. isonovalal, optimization studies of synthesis
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
i.e. isonovalal, optimization studies of synthesis
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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both rings of pinene are cleaved
i.e. isonovalal, optimization studies of synthesis
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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i.e. isonovalal, optimization studies of synthesis
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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enzyme is involved in the pathway of the metabolism of alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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enzyme is involved in the pathway of the metabolism of alpha-pinene
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
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additional information
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enzyme is devoid of prosthetic groups and has no cofactor requirement
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additional information
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no cofactor requirements
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(Z)-2-Methyl-5-isopropylhexa-2,5-dienal
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irreversible inactivation
5,5'-dithiobis(2-nitrobenzoate)
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p-hydroxymercuribenzoate
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Pinane derivatives with substituent groups at carbon 3
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Sulfhydryl reactive compounds
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0.009
alpha-Pinene oxide
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pH 9.0, 30°C
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250
alpha-Pinene oxide
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pH 9.0, 30°C
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120
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after 13fold purification
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7 - 10
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no significant variation in activity over this range
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5.2
calculated from amino acid sequence
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wild-type and mutant cultures
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wild-type and mutant cultures
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and strain NCIB 11671
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and strain NCIB 11671
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UniProt
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UniProt
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A0A0U2U334_9PSED
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23620
TrEMBL
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17000
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x * 17000 + x * 22000, SDS-PAGE
22000
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x * 17000 + x * 22000, SDS-PAGE
50000
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ultracentrifugal analysis
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x * 17000 + x * 22000, SDS-PAGE
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x * 17000 + x * 22000, SDS-PAGE
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x * 23700, calculated from amino acid sequence
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x * 25000, SDS-PAGE
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x * 23700, calculated from amino acid sequence
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inactivation of purified enzyme is mainly due to its direct contact with the hexadecane/water interface leading to precipitation
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retains activity after prolonged dialysis
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additional information
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high stability of enzyme system towards organic solvent
additional information
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high stability of enzyme system towards organic solvent
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4°C, 10 days, 7% loss of activity
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DEAE-Sephacel column chromatography and Superdex 75 gel filtration
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Ni-NTA column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(lambdDE3) cells
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Griffiths, E.T.; Harries, P.C.; Jeffcoat, R.; Trudgill, P.W.
Purification and properties of a alpha-pinene oxide lyase from Nocardia sp. strain P18.3
J. Bacteriol.
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4980-4983
1987
Nocardia sp., Nocardia sp. P18.3
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Griffiths, E.T.; Bociek, S.M.; Harries, P.C.; Jeffcoat, R.; Sissons, D.J.; Trudgill, P.W.
Bacterial metabolism of alpha-pinene: pathway from alpha-pinene oxide to acyclic metabolites in Nocardie sp. strain P18.3
J. Bacteriol.
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4972-4979
1987
Nocardia sp., Pseudomonas sp., Nocardia sp. P18.3, Pseudomonas sp. NCIB 10684
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Colocousi, A.; Saqib, K.M.; Leak, D.J.
Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of alpha-pinene
Appl. Microbiol. Biotechnol.
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822-830
1996
Pseudomonas fluorescens, Pseudomonas fluorescens NCIMB 11671
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Fontanille, P.; Le Fleche, A.; Larroche, C.
Pseudomonas rhodesiae PF1: A new and efficient biocatalyst for production of isonovalal from alpha-pinene oxide
Biocatal. Biotransform.
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413-421
2002
Pseudomonas rhodesiae, Pseudomonas rhodesiae PF1
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Fontanille, P.; Larroche, C.
Optimization of isonovalal production from alpha-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491
Appl. Microbiol. Biotechnol.
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534-540
2003
Pseudomonas rhodesiae, Pseudomonas rhodesiae PF1
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Linares, D.; Fontanille, P.; Larroche, C.
Protective effect of biomass components against interfacial inactivation of alpha-pinene oxide lyase from Pseudomonas rhodesiae CIP 107491
Food Technol. Biotechnol.
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202-207
2008
Pseudomonas rhodesiae, Pseudomonas rhodesiae CIP 107491
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Dubessay, P.; Larroche, C.; Fontanille, P.
Cloning and characterization of the gene encoding alpha-pinene oxide lyase enzyme (Pralpha-POL) from Pseudomonas rhodesiae CIP 107491 and production of the recombinant protein in Escherichia coli
Appl. Biochem. Biotechnol.
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676-690
2018
Pseudomonas rhodesiae (A0A0U2U334), Pseudomonas rhodesiae, Pseudomonas rhodesiae CIP 107491 (A0A0U2U334), Pseudomonas rhodesiae CIP 107491
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5.5.1.10 alpha-pinene-oxide decyclase
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