Information on EC 5.5.1.10 - alpha-pinene-oxide decyclase

for references in articles please use BRENDA:EC5.5.1.10
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
5.5.1.10
-
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RECOMMENDED NAME
GeneOntology No.
alpha-pinene-oxide decyclase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase reaction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Limonene and pinene degradation
-
-
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SYSTEMATIC NAME
IUBMB Comments
alpha-pinene-oxide lyase (decyclizing)
Both rings of pinene are cleaved in the reaction.
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CAS REGISTRY NUMBER
COMMENTARY hide
112692-50-9
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
wild-type and mutant cultures
-
-
Manually annotated by BRENDA team
wild-type and mutant cultures
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-
Manually annotated by BRENDA team
and strain NCIB 11671
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-
Manually annotated by BRENDA team
and strain NCIB 11671
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Z)-2-Methyl-5-isopropylhexa-2,5-dienal
-
irreversible inactivation
5,5'-dithiobis(2-nitrobenzoate)
-
-
beta-Pinene oxide
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-
Carvone
-
-
limonene oxide
-
-
p-hydroxymercuribenzoate
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-
Pinan-3-ol
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-
Pinan-3-one
-
-
Pinane derivatives with substituent groups at carbon 3
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-
Sodium arsenite
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weak
Sulfhydryl reactive compounds
-
-
-
Terpene epoxides
-
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
alpha-pinene
-
pH 9.0, 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
alpha-pinene
-
pH 9.0, 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
crude extract
120
-
after 13fold purification
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
no significant variation in activity over this range
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoelectric focusing
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
-
x * 17000 + x * 22000, SDS-PAGE
22000
-
x * 17000 + x * 22000, SDS-PAGE
50000
-
ultracentrifugal analysis
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation of purified enzyme is mainly due to its direct contact with the hexadecane/water interface leading to precipitation
-
retains activity after prolonged dialysis
-
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 10 days, 7% loss of activity
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography and Superdex 75 gel filtration
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strain P18.3
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LINKS TO OTHER DATABASES (specific for EC-Number 5.5.1.10)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)