Information on EC 5.5.1.10 - alpha-pinene-oxide decyclase

Substrates: both rings of pinene are cleaved
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: both rings of pinene are cleaved
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: enzyme is involved in the pathway of the metabolism of alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: enzyme is involved in the pathway of the metabolism of alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: i.e. isonovalal, optimization studies of synthesis

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: both rings of pinene are cleaved
Products: i.e. isonovalal, optimization studies of synthesis

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: both rings of pinene are cleaved
Products: i.e. isonovalal, optimization studies of synthesis

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: i.e. isonovalal, optimization studies of synthesis

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Pseudomonas sp.

Pseudomonas sp. NCIB 10684

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

6 entries

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: alpha-pinene oxide is an intermediate in the degradation of alpha-pinene by Nocardia sp. strain P18.3 and some Pseudomonas strains, inducible by growth with alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: enzyme is involved in the pathway of the metabolism of alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: enzyme is involved in the pathway of the metabolism of alpha-pinene
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

alpha-pinene oxide

(Z)-2-methyl-5-isopropylhexa-2,5-dienal

Substrates: -
Products: -

show all columns Go to Cofactor Search

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

additional information

additional information

enzyme is devoid of prosthetic groups and has no cofactor requirement

additional information

no cofactor requirements

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(Z)-2-Methyl-5-isopropylhexa-2,5-dienal

irreversible inactivation

show all columns Go to KM Value Search

5,5'-dithiobis(2-nitrobenzoate)

beta-Pinene oxide

Carvone

limonene oxide

p-hydroxymercuribenzoate

Pinan-3-ol

Pinan-3-one

Pinane derivatives with substituent groups at carbon 3

sodium arsenite

weak

Sulfhydryl reactive compounds

Terpene epoxides

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.009

alpha-Pinene oxide

pH 9.0, 30°C

show all columns Go to Turnover Number Search

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

250

alpha-Pinene oxide

pH 9.0, 30°C

show all columns Go to Specific Activity Search

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

120

after 13fold purification

312

crude extract

show all columns Go to pH Optimum Search

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

7 - 10

no significant variation in activity over this range

show all columns Go to Temperature Optimum Search

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

assay at

show all columns Go to Pi Value Search

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pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

isoelectric focusing

5.2

calculated from amino acid sequence

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

brenda

wild-type and mutant cultures

brenda

wild-type and mutant cultures

brenda

648896, 648897

brenda

Pseudomonas sp.

and strain NCIB 11671

Pseudomonas sp. NCIB 10684

brenda

and strain NCIB 11671

brenda

648896, 648897, 692433

brenda

UniProt

brenda

brenda

UniProt

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

8 entries

648893, 648894

brenda

brenda

brenda

brenda

brenda

brenda

Pseudomonas sp.

brenda

Pseudomonas sp. NCIB 10684

brenda

show all columns Go to Localization Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

A0A0U2U334 pBLAST

A0A0U2U334_9PSED

202

23620

TrEMBL

Show Sequence

show all columns Go to Molecular Weight Search

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

17000

x * 17000 + x * 22000, SDS-PAGE

22000

x * 17000 + x * 22000, SDS-PAGE

50000

ultracentrifugal analysis

show all columns Go to Subunit Search

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6 entries

x * 17000 + x * 22000, SDS-PAGE

x * 17000 + x * 22000, SDS-PAGE

x * 23700, calculated from amino acid sequence

x * 25000, SDS-PAGE

x * 23700, calculated from amino acid sequence

x * 25000, SDS-PAGE

Go to General Stability Search

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

inactivation of purified enzyme is mainly due to its direct contact with the hexadecane/water interface leading to precipitation

retains activity after prolonged dialysis

show all columns Go to Organic Solvent Search

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

additional information

high stability of enzyme system towards organic solvent

additional information

high stability of enzyme system towards organic solvent

Go to Storage Stability Search

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

4°C, 10 days, 7% loss of activity

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

DEAE-Sephacel column chromatography and Superdex 75 gel filtration

Ni-NTA column chromatography, and Superdex 200 gel filtration

Go to Cloned (commentary) Search

strain P18.3

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21(lambdDE3) cells

top print hide References Search

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

Griffiths, E.T.; Harries, P.C.; Jeffcoat, R.; Trudgill, P.W.

Purification and properties of a alpha-pinene oxide lyase from Nocardia sp. strain P18.3

J. Bacteriol.

169

4980-4983

1987

Nocardia sp., Nocardia sp. P18.3

3667522

brenda

Griffiths, E.T.; Bociek, S.M.; Harries, P.C.; Jeffcoat, R.; Sissons, D.J.; Trudgill, P.W.

Bacterial metabolism of alpha-pinene: pathway from alpha-pinene oxide to acyclic metabolites in Nocardie sp. strain P18.3

J. Bacteriol.

169

4972-4979

1987

Nocardia sp., Pseudomonas sp., Nocardia sp. P18.3, Pseudomonas sp. NCIB 10684

3667521

brenda

Colocousi, A.; Saqib, K.M.; Leak, D.J.

Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of alpha-pinene

Appl. Microbiol. Biotechnol.

822-830

1996

Pseudomonas fluorescens, Pseudomonas fluorescens NCIMB 11671

brenda

Fontanille, P.; Le Fleche, A.; Larroche, C.

Pseudomonas rhodesiae PF1: A new and efficient biocatalyst for production of isonovalal from alpha-pinene oxide

Biocatal. Biotransform.

413-421

2002

Pseudomonas rhodesiae, Pseudomonas rhodesiae PF1

brenda

Fontanille, P.; Larroche, C.

Optimization of isonovalal production from alpha-pinene oxide using permeabilized cells of Pseudomonas rhodesiae CIP 107491

Appl. Microbiol. Biotechnol.

534-540

2003

Pseudomonas rhodesiae, Pseudomonas rhodesiae PF1

12536252

brenda

Linares, D.; Fontanille, P.; Larroche, C.

Protective effect of biomass components against interfacial inactivation of alpha-pinene oxide lyase from Pseudomonas rhodesiae CIP 107491

Food Technol. Biotechnol.

202-207

2008

Pseudomonas rhodesiae, Pseudomonas rhodesiae CIP 107491

brenda