Information on EC 5.5.1.10 - alpha-pinene-oxide decyclase

for references in articles please use BRENDA:EC5.5.1.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
5.5.1.10
-
top print hide
RECOMMENDED NAME
GeneOntology No.
alpha-pinene-oxide decyclase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase reaction
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Limonene and pinene degradation
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
alpha-pinene-oxide lyase (decyclizing)
Both rings of pinene are cleaved in the reaction.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
112692-50-9
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain P18.3
-
-
Manually annotated by BRENDA team
strain P18.3
-
-
Manually annotated by BRENDA team
strain NCIMB 11671, wild-type and mutant cultures
-
-
Manually annotated by BRENDA team
strain NCIMB 11671, wild-type and mutant cultures
-
-
Manually annotated by BRENDA team
strain CIP 107491
-
-
Manually annotated by BRENDA team
strain PF1
-
-
Manually annotated by BRENDA team
strain NCIB 10684, NCIB 11671
-
-
Manually annotated by BRENDA team
strain NCIB 10684, NCIB 11671
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Z)-2-Methyl-5-isopropylhexa-2,5-dienal
-
irreversible inactivation
5,5'-dithiobis(2-nitrobenzoate)
-
-
beta-Pinene oxide
-
-
Carvone
-
-
limonene oxide
-
-
p-hydroxymercuribenzoate
-
-
Pinan-3-ol
-
-
Pinan-3-one
-
-
Pinane derivatives with substituent groups at carbon 3
-
-
Sodium arsenite
-
weak
Sulfhydryl reactive compounds
-
-
-
Terpene epoxides
-
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
alpha-pinene
-
pH 9.0, 30°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
alpha-pinene
-
pH 9.0, 30°C
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
crude extract
120
-
after 13fold purification
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
no significant variation in activity over this range
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
top print hide
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoelectric focusing
top print hide
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
-
x * 17000 + x * 22000, SDS-PAGE
22000
-
x * 17000 + x * 22000, SDS-PAGE
50000
-
ultracentrifugal analysis
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation of purified enzyme is mainly due to its direct contact with the hexadecane/water interface leading to precipitation
-
retains activity after prolonged dialysis
-
top print hide
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 10 days, 7% loss of activity
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography and Superdex 75 gel filtration
-
strain P18.3
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 5.5.1.10)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)