Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Synonyms
2,3-Oxidosqualene cyclase, OSC, OsOS, OsOSC7, pentacyclic orysatinol synthase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3S)-2,3-epoxy-2,3-dihydrosqualene = orysatinol
(3S)-2,3-epoxy-2,3-dihydrosqualene = orysatinol
reaction from bicyclic C8-cation (chair-boat form) via orysatinyl C-17 cation (chair-chair-chair form) to orysatinol, proposed reaction scheme of cyclization, overview
(3S)-2,3-epoxy-2,3-dihydrosqualene = orysatinol
reaction from bicyclic C8-cation (chair-boat form) via orysatinyl C-17 cation (chair-chair-chair form) to orysatinol, proposed reaction scheme of cyclization, overview
Oryza sativa Japonica Group Guangluai 15
-
-
(3S)-2,3-epoxy-2,3-dihydrosqualene = orysatinol
reaction from bicyclic C8-cation (chair-boat form) via orysatinyl C-17 cation (chair-chair-chair form) to orysatinol, proposed reaction scheme of cyclization, overview
Oryza sativa Japonica Group Zhonghua 11
-
-
(3S)-2,3-epoxy-2,3-dihydrosqualene = orysatinol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(3S)-2,3-epoxy-2,3-dihydrosqualene
orysatinol
-
-
-
?
additional information
?
-
additional information
?
-
identification of compounds by GC-MS analysis
-
-
?
additional information
?
-
Oryza sativa Japonica Group Guangluai 15
identification of compounds by GC-MS analysis
-
-
?
additional information
?
-
Oryza sativa Japonica Group Zhonghua 11
-
identification of compounds by GC-MS analysis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
identification of three key amino acid residues amongst 46 polymorphic sites that determine functional conversion between tetracyclic parkeol synthase (OsPS) and pentacyclic orysatinol synthase (OsOS), specifically, the chair-semi(chair)-chair and chair-boat-chair interconversions. Mechanism for the function conversion between OsPS and OsOS. The different orientation of a fourth amino acid residue Y257 is important for functional conversion. Three-dimensional modeling of the enzyme proteins generated by using Homo sapiens LAS structure (PDB ID 1W6K) as a template. Ligand docking
evolution
molecular evolutionary analysis. Phylogenetic, network and functional analyses of OsOSC7 variants in Oryza sativa subsp. japonica and in Oryza wild-type species, overview
evolution
Oryza sativa Japonica Group Guangluai 15
-
molecular evolutionary analysis. Phylogenetic, network and functional analyses of OsOSC7 variants in Oryza sativa subsp. japonica and in Oryza wild-type species, overview
-
evolution
Oryza sativa Japonica Group Zhonghua 11
-
molecular evolutionary analysis. Phylogenetic, network and functional analyses of OsOSC7 variants in Oryza sativa subsp. japonica and in Oryza wild-type species, overview
-
physiological function
promiscuous OSC from Oryza sativa (OsOS) synthesizes a pentacyclic triterpene orysatinol as its main product. The OsOS gene is widely distributed in indica subspecies of cultivated rice and in wild rice accessions
physiological function
Oryza sativa Japonica Group Guangluai 15
-
promiscuous OSC from Oryza sativa (OsOS) synthesizes a pentacyclic triterpene orysatinol as its main product. The OsOS gene is widely distributed in indica subspecies of cultivated rice and in wild rice accessions
-
physiological function
Oryza sativa Japonica Group Zhonghua 11
-
promiscuous OSC from Oryza sativa (OsOS) synthesizes a pentacyclic triterpene orysatinol as its main product. The OsOS gene is widely distributed in indica subspecies of cultivated rice and in wild rice accessions
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A732I
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, the mutant produces parkeol (16.8% of triterpenes) and a small amount of orysatinol (8.7% of triterpenes), compared to wild-type enzyme OsOS
L124F
site-directed mutagenesis,the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
L124F/A732I
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
L124F/L365F
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
L124F/L365F/A732I
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
L365F
site-directed mutagenesis, the mutation causes decreased orysatinol production (43.7% of of triterpenes) with concomitant increases in other non-parkeol triterpenes (56.3% of triterpenes)
A732I
Oryza sativa Japonica Group Guangluai 15
-
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, the mutant produces parkeol (16.8% of triterpenes) and a small amount of orysatinol (8.7% of triterpenes), compared to wild-type enzyme OsOS
-
L124F
Oryza sativa Japonica Group Guangluai 15
-
site-directed mutagenesis,the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
-
L365F
Oryza sativa Japonica Group Guangluai 15
-
site-directed mutagenesis, the mutation causes decreased orysatinol production (43.7% of of triterpenes) with concomitant increases in other non-parkeol triterpenes (56.3% of triterpenes)
-
A732I
Oryza sativa Japonica Group Zhonghua 11
-
site-directed mutagenesis, the mutant acquires parkeol synthase activity, EC 5.4.99.47, the mutant produces parkeol (16.8% of triterpenes) and a small amount of orysatinol (8.7% of triterpenes), compared to wild-type enzyme OsOS
-
L124F
Oryza sativa Japonica Group Zhonghua 11
-
site-directed mutagenesis,the mutant acquires parkeol synthase activity, EC 5.4.99.47, whereas the synthesis of orysatinol is reduced in comparison to wild-type OsOS
-
L365F
Oryza sativa Japonica Group Zhonghua 11
-
site-directed mutagenesis, the mutation causes decreased orysatinol production (43.7% of of triterpenes) with concomitant increases in other non-parkeol triterpenes (56.3% of triterpenes)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Xue, Z.; Tan, Z.; Huang, A.; Zhou, Y.; Sun, J.; Wang, X.; Thimmappa, R.B.; Stephenson, M.J.; Osbourn, A.; Qi, X.
Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species
New Phytol.
218
1076-1088
2018
Oryza sativa Japonica Group (H2KWF1), Oryza sativa Japonica Group Guangluai 15 (H2KWF1)
brenda