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Information on EC 5.4.99.65 - pre-alpha-onocerin synthase and Organism(s) Lycopodium clavatum and UniProt Accession A0A125SXN1

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.65 pre-alpha-onocerin synthase
IUBMB Comments
Isolated from the plant Lycopodium clavatum. The enzyme does not act on (3S)-2,3-epoxy-2,3-dihydrosqualene and does not form any alpha-onocerin.
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This record set is specific for:
Lycopodium clavatum
UNIPROT: A0A125SXN1
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The taxonomic range for the selected organisms is: Lycopodium clavatum
The expected taxonomic range for this enzyme is: Lycopodium clavatum
Synonyms
Lcc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Lcc
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(3S,22S)-2,3:22,23-diepoxy-2,3,22,23-tetrahydrosqualene mutase (cyclizing, pre-alpha-onocerin-forming)
Isolated from the plant Lycopodium clavatum. The enzyme does not act on (3S)-2,3-epoxy-2,3-dihydrosqualene and does not form any alpha-onocerin.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S,22S)-2,3:22,23-diepoxy-2,3,22,23-tetrahydrosqualene
pre-alpha-onocerin
show the reaction diagram
-
-
-
?
2,3,22,23-dioxidosqualene
pre-alpha-onocerin
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3S,22S)-2,3:22,23-diepoxy-2,3,22,23-tetrahydrosqualene
pre-alpha-onocerin
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
ferns are known to produce onoceroids including onoceranes and serratanes having unusual structures among triterpenes. Serratanes are directly biosynthesized from pre-alpha-onocerin, synthesized by pre-alpha-onocerin synthase, and not from alpha-onocerin, synthesized by alpha-onocerin synthase, EC 5.4.99.66, from pre-alpha-onocerin
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LCC_LYCCL
756
2
86189
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
while the heterologous LCC gene expression produces the bicyclic triterpene pre-alpha-onocerin, a combination of LCC and alpha-onocerin synthase LCD produces onoceranes, and that of LCC and tohogenol synthase LCE produces serratanes
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
functional recombinant enzyme expression in Saccharomyces cerevisiae strain GIL77, coexpression with alpha-onocerin synthase leading to the production of tohogenol and serratenediol, NMR metabolite identification, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Araki, T.; Saga, Y.; Marugami, M.; Otaka, J.; Araya, H.; Saito, K.; Yamazaki, M.; Suzuki, H.; Kushiro, T.
Onocerin biosynthesis requires two highly dedicated triterpene cyclases in a fern Lycopodium clavatum
ChemBioChem
17
288-290
2016
Lycopodium clavatum (A0A125SXN1)
-
Manually annotated by BRENDA team
Saga, Y.; Araki, T.; Araya, H.; Saito, K.; Yamazaki, M.; Suzuki, H.; Kushiro, T.
Identification of serratane synthase gene from the fern Lycopodium clavatum
Org. Lett.
19
496-499
2017
Lycopodium clavatum (A0A125SXN1)
Manually annotated by BRENDA team