cytoplasmic and mitochondrial tRNA(Met), mitochondrial tRNA(Lys) and tRNA(Ser). Recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates. The enzyme does not catalyse the pseudouridylation of uridine31
the deletion mutant strain DELTAYGR169c shows no detectable growth phenotype. The only difference in pseudouridine formation in stable cellular RNAs is the absence of pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs. Complementation of the DELTAYGR169c strain by a plasmid bearing the wild-type YGR169c ORF restores pseudouridine31 formation in tRNA
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UNIPROT
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recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates
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5.4.99.42 tRNA pseudouridine31 synthase
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