although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
gene MdOSC4 encodes a multifunctional oxidosqualene cyclase producing an oleanane-type triterpene, putatively identified as germanicol, as well as beta-amyrin and lupeol, in the proportion 82:14:4
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
although the MdOSC4 gene was expressed in the skins of all apple varieties under investigation, no germanicol or derivatives were detected at the metabolite level
MdOSC4 contains the highly conserved DCTAE motif, which is implicated in substrate binding, and six repeats of the QW motifs, typical of the triterpene synthase superfamily, sequence comparisons and phylogenetic analysis
the enzyme is involved in the biosynthesis of pentacyclic triterpenes, which, in apple, account for 32-70% of the epicuticular waxes, depending on the cultivar
analysis of triterpenes from apple fruits in a collection of 20 contrasting apple cultivars, expression patterns of triterpene-related genes in different apple cultivars, overview
gene OSC4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis of the enzyme in apple fruits, functional recombinant expression in Nicotiana benthamiana via transformation by Agrobacterium tumefaciens strain GV3101 resulting in production of germanicol, beta-amyrin, and lupeol
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Enzyme Nomenclature
5.4.99.34 germanicol synthase
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