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Information on EC 5.4.99.30 - UDP-arabinopyranose mutase and Organism(s) Oryza sativa and UniProt Accession Q6Z4G3

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.30 UDP-arabinopyranose mutase
IUBMB Comments
The reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) .
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This record set is specific for:
Oryza sativa
UNIPROT: Q6Z4G3
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Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
udp-arabinopyranose mutase, cruam, osuam3, atrgp2, bdrgp1, atrgp1, osuam1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Os07g0604800 protein
-
Os03g0599800 protein
-
Os03g40270 protein
-
Os07g41360 protein
-
OsUAM
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose
show the reaction diagram
in functional UDP-arabinopyranose mutases a specific arginyl residue is reversibly glycosylated. This arginyl residue together with a DXD motif is required for catalytic activity
UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose
show the reaction diagram
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
UDP-arabinopyranose pyranomutase
The reaction is reversible and at thermodynamic equilibrium the pyranose form is favored over the furanose form (90:10) [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
show the reaction diagram
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
show the reaction diagram
UDP-beta-L-arabinopyranose
UDP-beta-L-arabinofuranose
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-beta-L-arabinofuranose
UDP-beta-L-arabinopyranose
show the reaction diagram
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
5 mM, activity almost doubles
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
5 mM, complete inhibition
UDP
competitive inhibition, lower affinity for enzyme than substrate
UDP-Gal
UDP-Glc
UDP-Xyl
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0228
UDP-beta-L-arabinofuranose
pH 6.8, 25°C
0.0454
UDP-beta-L-arabinopyranose
pH 6.8, 25°C
0.0228 - 0.0531
UDP-beta-L-arabinofuranose
0.0454 - 0.055
UDP-beta-L-arabinopyranose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.474
UDP-beta-L-arabinofuranose
pH 6.8, 25°C
0.185
UDP-beta-L-arabinopyranose
pH 6.8, 25°C
0.474
UDP-beta-L-arabinofuranose
pH 6.8, 25°C
0.185
UDP-beta-L-arabinopyranose
pH 6.8, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.8
UDP-beta-L-arabinofuranose
pH 6.8, 25°C
40.8
UDP-beta-L-arabinopyranose
pH 6.8, 25°C
20.8
UDP-beta-L-arabinofuranose
pH 6.8, 25°C
40.8
UDP-beta-L-arabinopyranose
pH 6.8, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
UDP
pH 6.8, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
pyranose forming activity
6.5
furanose-forming activity
5.5 - 6
formation of beta-L-arabinofuranose
6
pyranose forming activity
6.5
furanose-forming activity
7 - 7.5
formation of beta-L-arabinopyranose
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
pH 4.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity, pyranose forming activity
5 - 8
pH 5.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity, furanose-forming activity
4 - 8
pH 4.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity, pyranose forming activity
5 - 8
pH 5.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity, furanose-forming activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 60
30°C: about 50% of maximal activity, 60°C: about 85% of maximal activity
30 - 60
30°C: about 50% of maximal activity, 60°C: about 85% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
RNAi is used to downregulate OsUAM gene expression. This leads to a reduction of between 6 and 44% in the amounts of arabinofuranose in the cell wall, a decrease in the extent of substitution of the xylan backbone, and a reduction of between 25% and 80% in the ferulic acid or p-coumaric acid contents of the wall. Transgenic rice plants with a >25% reduction in arabinose content are dwarfed and infertile
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, SDS-PAGE
41349
x * 41349, calculated from sequence
40000
x * 40000, SDS-PAGE
41000
x * 41000, SDS-PAGE
41349
x * 41349, calculated from sequence
460000
mutase is likely to exist as a complex composed of numerous proteins, gel filtration
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
autoglycosylation occurs at a specific arginyl residue in recombinant UDP-arabinopyranose mutase that is essential for mutase activity
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R149A
activity is 154.1% of wild-type activity
R156A
activity is 0.1% of wild-type activity
R163A
activity is 2.0% of wild-type activity
D112N
activity is 131% of wild-type activity
R151A
no activity
R158A
activity is 5.7% of wild-type activity
R165A
activity is 131% of wild-type activity
R165K
activity is 131% of wild-type activity
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
80 h, 50% loss of activity
50
1 h, 50% loss of activity
25
80 h, 50% loss of activity
50
1 h, 50% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
rUMA1 expressed in insect cells retains activity after repeated cycles of freezing and thawing
repeated cycles of freezing and thawing did not significantly affect mutase activity
rUMA1 expressed in insect cells retains activity after repeated cycles of freezing and thawing
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, mutase activity is stable for at least 3 months
4°C, mutase activity is stable for 3 weeks
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in insect cells
expressed in Escherichia coli
expression in insect cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Konishi, T.; Miyazaki, Y.; Yamakawa, S.; Iwai, H.; Satoh, S.; Ishii, T.
Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells
Biosci. Biotechnol. Biochem.
74
191-194
2010
Oryza sativa (Q6Z4G3), Oryza sativa (Q8H8T0), Oryza sativa
Manually annotated by BRENDA team
Konishi, T.; Ohnishi-Kameyama, M.; Funane, K.; Miyazaki, Y.; Konishi, T.; Ishii, T.
An arginyl residue in rice UDP-arabinopyranose mutase is required for catalytic activity and autoglycosylation
Carbohydr. Res.
345
787-791
2010
Oryza sativa (Q6Z4G3), Oryza sativa (Q8H8T0), Oryza sativa
Manually annotated by BRENDA team
Konishi, T.; Takeda, T.; Miyazaki, Y.; Ohnishi-Kameyama, M.; Hayashi, T.; O'Neill, M.A.; Ishii, T.
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
Glycobiology
17
345-354
2006
Oryza sativa (O82706), Oryza sativa (Q8H8T0), Oryza sativa
Manually annotated by BRENDA team
Konishi, T.; Aohara, T.; Igasaki, T.; Hayashi, N.; Miyazaki, Y.; Takahashi, A.; Hirochika, H.; Iwai, H.; Satoh, S.; Ishii, T.
Down-regulation of UDP-arabinopyranose mutase reduces the proportion of arabinofuranose present in rice cell walls
Phytochemistry
72
1962-1968
2011
Oryza sativa
Manually annotated by BRENDA team