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Information on EC 5.4.99.28 - tRNA pseudouridine32 synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q12069

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.28 tRNA pseudouridine32 synthase
IUBMB Comments
The dual-specificity enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA . cf. EC 5.4.99.29 (23S rRNA pseudouridine746 synthase).
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: Q12069
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
tRNA uridine32
=
tRNA pseudouridine32
Synonyms
rib2/pus8p, pseudouridine synthase rlua, trna:psi32-synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tRNA:Psi32-synthase
-
tRNA:Psi32-synthase
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine32 uracil mutase
The dual-specificity enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA [5]. cf. EC 5.4.99.29 (23S rRNA pseudouridine746 synthase).
CAS REGISTRY NUMBER
COMMENTARY hide
430429-15-5
-
61506-89-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine32
tRNA pseudouridine32
show the reaction diagram
tRNA uridine32
tRNA pseudouridine32
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine32
tRNA pseudouridine32
show the reaction diagram
Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs
-
-
?
tRNA uridine32
tRNA pseudouridine32
show the reaction diagram
Rib2/Pus8p acts specifically on cytoplasmic tRNAs, whereas Pus9p modifies the same position mostly in mitochondrial tRNAs
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium
malfunction
Rib2/Pus8p acts on cytoplasmic tRNAs, whereas Pus9p modifies the same position in mitochondrial tRNAs. The DELTArib2/pus8/DELTApus9, is viable only in the presence of 15 mg/liter riboflavin in the growth medium
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D112A
mutant does not pseudouridine32 in formation in cytoplasmic tRNAGly of the DELTArib2/pus8/DELTApus9 strain
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Behm-Ansmant, I.; Grosjean, H.; Massenet, S.; Motorin, Y.; Branlant, C.
Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae
J. Biol. Chem.
279
52998-53006
2004
Saccharomyces cerevisiae (Q12069), Saccharomyces cerevisiae (Q12362), Saccharomyces cerevisiae
Manually annotated by BRENDA team