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Information on EC 5.4.99.27 - tRNA pseudouridine13 synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q08647

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.27 tRNA pseudouridine13 synthase
IUBMB Comments
Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine13 in tRNA [2,3]. The Pus7 protein from Saccharomyces cerevisiae is a multisite-multisubstrate pseudouridine synthase that is able to modify uridine13 in several yeast tRNAs, uridine35 in the pre-tRNATyr, uridine35 in U2 small nuclear RNA, and uridine50 in 5S rRNA .
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Saccharomyces cerevisiae
UNIPROT: Q08647
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
tRNA uridine13
=
tRNA pseudouridine13
Synonyms
pus7p, pus7 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
RNA:pseudouridine-synthase Pus7p
-
RNA:PSI-synthase Pus7p
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine13 uracil mutase
Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine13 in tRNA [2,3]. The Pus7 protein from Saccharomyces cerevisiae is a multisite-multisubstrate pseudouridine synthase that is able to modify uridine13 in several yeast tRNAs, uridine35 in the pre-tRNATyr, uridine35 in U2 small nuclear RNA, and uridine50 in 5S rRNA [5].
CAS REGISTRY NUMBER
COMMENTARY hide
430429-15-5
-
61506-89-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine13
tRNA pseudouridine13
show the reaction diagram
tRNA uridine13
tRNA pseudouridine13
show the reaction diagram
-
Pus7p is required for inducible pseudouridylation at position 35 and 56 during nutrient deprivation or heat shock
-
-
?
additional information
?
-
for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus7-dependent sites at position 13 of glutamate tRNA. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine13
tRNA pseudouridine13
show the reaction diagram
-
-
-
?
additional information
?
-
for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus7-dependent sites at position 13 of glutamate tRNA. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
disruption of the PUS7 gene abolishes pseudouridine13 formation in tRNAs in vivo. Absence of pseudouridine13 formation in tRNAAsp and tRNAGlu extracted from the DELTAPUS7 strain
physiological function
the most abundant RNA modification is pseudouridine (Psi), Psi is ubiquitous in diverse RNAs, and dynamic in mRNA. Pseudouridylation profiles and mechanisms. Pus7-dependent induction of pseudouridylation during heat shock
malfunction
-
using a Pus7p mutant strain it is shown that Pus7p is responsible for inducible U2 pseudouridylation at position 56
metabolism
-
Pus7p that catalyses U2 pseudouridylation at position 35, also catalyses pseudouridylation at position 56 during nutrient deprivation or heat shock
additional information
transcriptome-wide quantitative mapping of Psi, method development using Psi-seq, relying on the unique stability of N3-[N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide-Psi] (N3-CMC-Psi) to alkaline hydrolysis, and the ability of N3-CMC-Psi to terminate reverse transcription. For rRNA, 24/24 Cbf5-dependent sites are known targets of pseudouridylation, 8/9 associations between snoRNAs and rRNA sites are known, as is the identified Pus5-mediated Psi site in mitochondrial 21S rRNA. One snR3-dependent site at position 2140 on 25S rRNA is not specificated, detailed overview. Cbf5-dependent-Psi sites in mRNAs and snoRNAs are likely snoRNA-guided
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transcriptome-wide quantitative mapping of Psi
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Urban, A.; Behm-Ansmant, I.; Branlant, C.; Motorlin, Y.
RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:PSI-synthase Pus7p
J. Biol. Chem.
284
5845-5858
2009
Saccharomyces cerevisiae (Q08647), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Behm-Ansmant, I.; Urban, A.; Ma, X.; Yu, Y.T.; Motorin, Y.; Branlant, C.
The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:PSI-synthase also acting on tRNAs
RNA
9
1371-1382
2003
Saccharomyces cerevisiae (Q08647), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Wu, G.; Xiao, M.; Yang, C.; Yu, Y.
U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP
EMBO J.
30
79-89
2011
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Schwartz, S.; Bernstein, D.A.; Mumbach, M.R.; Jovanovic, M.; Herbst, R.H.; Leon-Ricardo, B.X.; Engreitz, J.M.; Guttman, M.; Satija, R.; Lander, E.S.; Fink, G.; Regev, A.
Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA
Cell
159
148-162
2014
Saccharomyces cerevisiae (Q08647)
Manually annotated by BRENDA team