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Information on EC 5.4.99.25 - tRNA pseudouridine55 synthase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WHP7

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.25 tRNA pseudouridine55 synthase
IUBMB Comments
Pseudouridine synthase TruB from Escherichia coli specifically modifies uridine55 in tRNA molecules . The bifunctional archaeal enzyme also catalyses the pseudouridylation of uridine54 . It is not known whether the enzyme from Escherichia coli can also act on position 54 in vitro, since this position is occupied in Escherichia coli tRNAs by thymine.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WHP7
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Word Map
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
tRNA uridine55
=
tRNA pseudouridine55
Synonyms
pus10, acbf5, psi synthase, pseudouridine 55 synthase, pseudouridine synthase trub, trna pseudouridine synthase, psi55s, trna:pseudouridine-55 synthase, ynl292w, rna pseudouridine synthase trub, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
psi55 tRNA pseudouridine synthase
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine55 uracil mutase
Pseudouridine synthase TruB from Escherichia coli specifically modifies uridine55 in tRNA molecules [1]. The bifunctional archaeal enzyme also catalyses the pseudouridylation of uridine54 [6]. It is not known whether the enzyme from Escherichia coli can also act on position 54 in vitro, since this position is occupied in Escherichia coli tRNAs by thymine.
CAS REGISTRY NUMBER
COMMENTARY hide
430429-15-5
-
61506-89-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, structure of pseudouridine55 tRNA in two substrate-free forms, at 1.9 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chaudhuri, B.N.; Chan, S.; Perry, L.J.; Yeates, T.O.
Crystal structure of the apo forms of psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis. A hinge at the base of the catalytic cleft
J. Biol. Chem.
279
24585-24591
2004
Mycobacterium tuberculosis (P9WHP7), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WHP7)
Manually annotated by BRENDA team