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Information on EC 5.4.99.24 - 23S rRNA pseudouridine955/2504/2580 synthase for references in articles please use BRENDA:EC5.4.99.24Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Escherichia coli
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23S rRNA pseudouridine955/2504/2580 synthase
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23S rRNA uridine955/uridine2504/uridine2580 = 23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
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23S rRNA-uridine955/2504/2580 uracil mutase
The enzyme converts uridines at position 955, 2504 and 2580 of 23S rRNA to pseudouridines.
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pseudouridine synthases RluC
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RLuc
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YceC
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SwissProt
brenda
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brenda
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malfunction
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in the rluC- strain, susceptibility to some antibiotics increases dramatically. The most pronounced effects in comparison with the rluC+ control are observed with clindamycin (16-fold), linezolid (8-fold), and tiamulin (4-fold)
malfunction
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disruption of yceC does not affect the growth
malfunction
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an rluC- mutant shows some cold sensitivity
malfunction
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deletion of this gene and thus the absence of these three pseudouridine residues has no detectable effect on the exponential growth rate in either rich or minimal glucose medium at temperatures ranging from 24°C to 42°C
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23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
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23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
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23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
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23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
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additional information
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RluC is associates with a pre-50S ribosomal particle
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brenda
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Escherichia coli (strain K12)
Escherichia coli (strain K12)
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monomer
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analytical ultracentrifugation sedimentation velocity experiments show that RluC(92-319) is monomeric in solution
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a proteolytically derived fragment of the enzyme consisting of residues 89-319 retains catalytic activity. Crystals of this fragment, grown by precipitation with sodium acetate at pH 8.0, belong to space group P321, with unit-cell dimensions a = b = 97.1, c = 86.3 A and have two molecules in the crystallographic asymmetric unit. Crystals diffract X-rays to at least 2.3 A resolution and appear suitable for crystal structure determination
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crystals of the catalytic domain RluC(92-319) are grown at 20°C using the hanging drop method
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overexpressed in Escherichia coli
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additional information
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a proteolytically derived fragment of the enzyme consisting of residues 89-319 retains catalytic activity
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Corollo, D.; Blair-Johnson, M.; Conrad, J.; Friedler, T.; Sun, D.; Wang, L.; Ofengand, J.; Fenna, R.
Crystallisation and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli
Acta Crystallogr. Sect. D
55
302-304
1999
Escherichia coli (P0AA39)
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brenda
Toh, S.M.; Mankin, A.S.
An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors
J. Mol. Biol.
380
593-597
2008
Escherichia coli
brenda
Huang, L.; Ku, J.; Pookanjanatavip, M.; Gu, X.; Wang, D.; Greene, P.J.; Santi, D.V.
Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA
Biochemistry
37
15951-15957
1998
Escherichia coli
brenda
Mizutani, K.; Machida, Y.; Unzai, S.; Park, S.Y.; Tame, J.R.
Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli
Biochemistry
43
4454-4463
2004
Escherichia coli, Escherichia coli (P0AA39)
brenda
Jiang, M.; Sullivan, S.M.; Walker, A.K.; Strahler, J.R.; Andrews, P.C.; Maddock, J.R.
Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques
J. Bacteriol.
189
3434-3444
2007
Escherichia coli
brenda
Conrad, J.; Sun, D.; Englund, N.; Ofengand, J.
The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA
J. Biol. Chem.
273
18562-18566
1998
Escherichia coli (P0AA39)
brenda
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