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Information on EC 5.4.99.17 - squalene-hopene cyclase

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.17 squalene-hopene cyclase
IUBMB Comments
The enzyme also produces the cyclization product hopan-22-ol by addition of water (cf. EC 4.2.1.129, squalene-hopanol cyclase). Hopene and hopanol are formed at a constant ratio of 5:1.
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This record set is specific for:
UNIPROT: Q9X7V9
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
squalene-hopene cyclase, squalene hopene cyclase, aacshc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclase, squalene-hopanoid
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SHC
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
squalene mutase (cyclizing)
The enzyme also produces the cyclization product hopan-22-ol by addition of water (cf. EC 4.2.1.129, squalene-hopanol cyclase). Hopene and hopanol are formed at a constant ratio of 5:1.
CAS REGISTRY NUMBER
COMMENTARY hide
76600-69-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
citronellal
isopulegol
show the reaction diagram
activity of mutant F449C, not of the wild-type enzyme
i.e. 2-isopropenyl-5-methyl-cyclohexanol
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?
squalene
hop-22(29)-ene
show the reaction diagram
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-
?
additional information
?
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no activity with citronellal or isopulegol (2-isopropenyl-5-methyl-cyclohexanol)
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
squalene
hop-22(29)-ene
show the reaction diagram
-
-
-
?
additional information
?
-
no activity with citronellal or isopulegol (2-isopropenyl-5-methyl-cyclohexanol)
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q9X7V9_STRCO
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
680
0
75215
TrEMBL
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F438C
site-directed mutagenesis, the mutant does not perform interconversion of citronellal and isopulegol
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Siedenburg, G.; Breuer, M.; Jendrossek, D.
Prokaryotic squalene-hopene cyclases can be converted to citronellal cyclases by single amino acid exchange
Appl. Microbiol. Biotechnol.
97
1571-1580
2013
Bradyrhizobium japonicum, Rhodopseudomonas palustris, Teredinibacter turnerae, Syntrophobacter fumaroxidans (A0LJS0), Alicyclobacillus acidocaldarius (P33247), Zymomonas mobilis (P33990), Zymomonas mobilis (Q5NM88), Burkholderia ambifaria (Q0B2H5), Burkholderia ambifaria (Q0B5S3), Bacillus anthracis (Q81YD8), Streptomyces coelicolor (Q9X7V9), Acetobacter pasteurianus (YP3187836), Burkholderia ambifaria ATCC BAA-244 / AMMD (Q0B2H5), Burkholderia ambifaria ATCC BAA-244 / AMMD (Q0B5S3), Zymomonas mobilis ATCC 31821 (P33990), Zymomonas mobilis CP4 (Q5NM88), Alicyclobacillus acidocaldarius DSM 446 (P33247)
Manually annotated by BRENDA team