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Information on EC 5.4.99.11 - isomaltulose synthase
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5.4.99.11 isomaltulose synthaseIUBMB Comments
The enzyme simultaneously produces isomaltulose (6-O-alpha-D-glucopyranosyl-D-fructose) and smaller amounts of trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose) from sucrose.
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Word Map
- 5.4.99.11
- synthesis
- erwinia
- rhapontici
- palatinose
-
protaminobacter
-
2.4.1.1
- pantoea
-
1,4-alpha-d-glucan:orthophosphate
- molasses
- plymuthica
- food industry
-
vacuole-targeted
- dispersa
- whiteflies
- bemisia
- honeydew
The enzyme appears in viruses and cellular organisms
Synonyms
sucrose isomerase, siase, alpha-glucosyltransferase, isomaltulose synthase, trehalulose synthase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-Glucosyltransferase
-
-
-
-
Isomaltulose synthetase
-
-
-
-
Sucrose 6-glucosylmutase
-
-
-
-
Sucrose alpha-glucosyltransferase
-
-
-
-
Sucrose mutase
-
-
-
-
Synthase, isomaltulose
-
-
-
-
Trehalulose synthase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
Sucrose glucosylmutase
The enzyme simultaneously produces isomaltulose (6-O-alpha-D-glucopyranosyl-D-fructose) and smaller amounts of trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose) from sucrose.
CAS REGISTRY NUMBER
COMMENTARY
159940-49-5
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose
6-O-alpha-D-Glucopyranosyl-D-fructofuranose
Paraburkholderia acidicola
-
-
-
r
sucrose
isomaltulose + trehalulose + D-glucose
Paraburkholderia acidicola
MutB catalyzes the isomerization of sucrose to mainly trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose)
-
-
r
additional information
?
-
Paraburkholderia acidicola
the isomaltulose-producing enzyme from Pseudomonas mesoacidophila MX-45 shows the main product trehalulose. It is also named alpha-glucosyltransferase and is renamed trehalulose synthase
-
-
?
additional information
?
-
Paraburkholderia acidicola
the enzyme catalyzes the enzymatic rearrangement of the alpha-1,2 linkage between glucose and fructose to an alpha-1,6 linkage (producing isomaltulose) or alpha-1,4 linkage (producing trehalulose). In addition, the enzyme hydrolyzes sucrose to produce small amounts of glucose and fructose monosaccharides
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose
6-O-alpha-D-Glucopyranosyl-D-fructofuranose
Paraburkholderia acidicola
-
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0192
sucrose
Paraburkholderia acidicola
pH not specified in the publication, 20°C, native enzyme
0.03
sucrose
Paraburkholderia acidicola
pH and temperature not specified in the publication, recombinant enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
36
sucrose
Paraburkholderia acidicola
pH and temperature not specified in the publication, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
13.98
Paraburkholderia acidicola
substrate sucrose, pH not specified in the publication, 20°C, native enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Q2PS28_9BURK
Burkholderia ubonensis subsp. mesacidophila
584
0
66792
TrEMBL
-
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D442N
Paraburkholderia acidicola
the mutant favors the transfer reaction with an isomer preference for isomaltulose
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
Paraburkholderia acidicola
isomaltulose (alpha-D-glucopyranosyl-1,6-D fructofuranose) is a carbohydrate used as ideal sucrose substitute. Sucrose isomerase is widely used in industries for the production of isomaltulose. The enzyme catalyzes the isomerization of sucrose into isomaltulose and trehalulose and may hydrolyze sucrose to produce small amounts of glucose and fructose
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Watzlawick, H.; Mattes, R.
Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45
Appl. Environ. Microbiol.
75
7026-7036
2009
Paraburkholderia acidicola (Q2PS28), Paraburkholderia acidicola MX-45 (Q2PS28)
Hamerli, D.; Birch, R.G.
Transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane
Plant Biotechnol. J.
9
32-37
2011
Paraburkholderia acidicola (Q2PS28), Paraburkholderia acidicola MX-45 (Q2PS28)
Mu, W.; Li, W.; Wang, X.; Zhang, T.; Jiang, B.
Current studies on sucrose isomerase and biological isomaltulose production using sucrose isomerase
Appl. Microbiol. Biotechnol.
98
6569-6582
2014
Erwinia rhapontici, Erwinia rhapontici (D9MPF2), Erwinia rhapontici (Q6XNK5), Erwinia rhapontici (Q9AI64), Serratia plymuthica, no activity in Bemisia argentifolii, Erwinia sp. D12, Enterobacter sp. FMB-1 (B5ABD8), Rhizobium sp. MX-45 (M1E1F6), Paraburkholderia acidicola (Q2PS28), Klebsiella pneumoniae (Q4L2Q1), Raoultella planticola (Q6XKX6), Pantoea dispersa (Q6XNK6), Klebsiella sp. LX3 (Q8KR84), Erwinia rhapontici DSM 4484 (Q9AI64), Klebsiella pneumoniae NK33-98-8 (Q4L2Q1), Raoultella planticola UQ14S (Q6XKX6), Erwinia rhapontici NCPPB 1578, Pantoea dispersa UQ68J (Q6XNK6), Serratia plymuthica CBS 574.77, Erwinia rhapontici wac2928 (Q6XNK5), Paraburkholderia acidicola MX-45 (Q2PS28), Serratia plymuthica ATCC 15928, Erwinia rhapontici NX-5 (D9MPF2)
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