Information on EC 5.4.99.11 - Isomaltulose synthase and Organism(s) Rhizobium sp. MX-45 and UniProt Accession M1E1F3

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This record set is specific for:
Rhizobium sp. MX-45
UNIPROT: M1E1F3


The taxonomic range for the selected organisms is: Rhizobium sp. MX-45

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.11
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RECOMMENDED NAME
GeneOntology No.
Isomaltulose synthase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
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SYSTEMATIC NAME
IUBMB Comments
Sucrose glucosylmutase
The enzyme simultaneously produces isomaltulose (6-O-alpha-D-glucopyranosyl-D-fructose) and smaller amounts of trehalulose (1-O-alpha-D-glucopyranosyl-beta-D-fructose) from sucrose.
CAS REGISTRY NUMBER
COMMENTARY hide
159940-49-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Pseudomonas mesoacidophila MX-45, gene mutB
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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the enzyme belongs to the glycoside hydrolase family 13 (GH13) in the CAZy classification
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additional information
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active site structure and substrate and ligand binding structures, overview
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sucrose
6-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
show the reaction diagram
additional information
?
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M1E1F6;
the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sucrose
6-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
show the reaction diagram
additional information
?
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M1E1F6
the enzyme catalyzes the isomerization of sucrose (alpha-D-glucosylpyranosyl-1,2-beta-D-fructofuranose) to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as the main products, and produces glucose and fructose in residual amounts because of sucrose hydrolysis
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F164L
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site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics
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R284C
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site-directed mutagenesis, three-dimensional structure from crystal structure analysis, comparison to the wild-type. The mutant shows hydrolytic activity converting sucrose to glucose and fructose, kinetics. Presence of Mg2+, Ca2+ and Zn2+ ions and glucose have no effect on the activity of the R284C mutant
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry
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sucrose isomerase activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. The industrial potential may be further enhanced by selection for variants that do not catabolize the sucrose substrate
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