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Information on EC 5.4.2.8 - phosphomannomutase and Organism(s) Arabidopsis thaliana and UniProt Accession O80840

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.2 Phosphotransferases (phosphomutases)
                5.4.2.8 phosphomannomutase
IUBMB Comments
alpha-D-Mannose 1,6-bisphosphate or alpha-D-glucose 1,6-bisphosphate can act as cofactor.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O80840
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
pmm, phosphomannomutase, orf17, phosphomannomutase 2, phosphomannomutase2, alpha-d-phosphohexomutase, phosphomannose mutase, pmm-1, pmm-2, alpha-phosphomannomutase1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphomannomutase
-
ORF17
-
-
-
-
Phosphomannose mutase
-
-
-
-
Phosphomutase, mannose
-
-
-
-
PMMH-22
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-mannose 1,6-phosphomutase
alpha-D-Mannose 1,6-bisphosphate or alpha-D-glucose 1,6-bisphosphate can act as cofactor.
CAS REGISTRY NUMBER
COMMENTARY hide
59536-73-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucose 1-phosphate
D-glucose 6-phosphate
show the reaction diagram
reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate
-
-
?
D-Mannose 1-phosphate
D-Mannose 6-phosphate
show the reaction diagram
reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate
-
-
r
D-mannose 6-phosphate
D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
alpha-D-mannose 1-phosphate
alpha-D-mannose 6-phosphate
show the reaction diagram
-
-
-
-
r
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-Glucose 1,6-bisphosphate
in presence of 0.02 and 0.2 mM mannose 1-phosphate substrate, half-maximal stimulation of recombinant enzyme at 0.004 and 0.008 mM D-glucose 1,6-bisphosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0654
D-glucose 1-phosphate
30°C, pH 7.5
0.0297
D-mannose 1-phosphate
30°C, pH 7.5
0.068 - 0.475
alpha-D-mannose 1-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 10.31
alpha-D-mannose 1-phosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
95% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
enzyme is constitutively expressed in both vegetative and reproductive organs
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PMM_ARATH
246
0
27762
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27700
x * 27700, calculated and MALDI-TOF
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27700, calculated and MALDI-TOF
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G7R
-
mutant exhibits activity similar to wild type enzyme
G7R/R37Q
-
temperature-sensitive Arabidopsis thaliana PMM-12 mutant (G7R/R37Q) has lower PMM protein and enzyme activity levels than the wild type enzyme
R37Q
-
mutant exhibits decreased activity similar to wild type enzyme
additional information
expression as green fluorescent protein fusion protein in Arabidospsis thaliana increases ascorbic acid content by 25-33%
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Talon metal affinity resin chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Agrobacterium tumefaciens
expression in Escherichia coli with His-tag, expression as green fluorescent protein fusion protein in Arabidospsis thaliana
expressed in Escherichia coli strain BL21(DE3)pLysE cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Qian, W.; Yu, C.; Qin, H.; Liu, X.; Zhang, A.; Johansen, I.E.; Wang, D.
Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana
Plant J.
49
399-413
2007
Nicotiana benthamiana, Arabidopsis thaliana (O80840)
Manually annotated by BRENDA team
Hoeberichts, F.A.; Vaeck, E.; Kiddle, G.; Coppens, E.; van de Cotte, B.; Adamantidis, A.; Ormenese, S.; Foyer, C.H.; Zabeau, M.; Inze, D.; Perilleux, C.; Van Breusegem, F.; Vuylsteke, M.
A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death
J. Biol. Chem.
283
5708-5718
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (O80840)
Manually annotated by BRENDA team