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Information on EC 5.4.1.3 - 2-methylfumaryl-CoA isomerase and Organism(s) Chloroflexus aurantiacus and UniProt Accession A9WC36

for references in articles please use BRENDA:EC5.4.1.3
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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.1 Transferring acyl groups
                5.4.1.3 2-methylfumaryl-CoA isomerase
IUBMB Comments
The enzyme, purified from the bacterium Chloroflexus aurantiacus, acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate. It is part of the 3-hydroxypropanoate cycle for carbon assimilation.
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Chloroflexus aurantiacus
UNIPROT: A9WC36
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The taxonomic range for the selected organisms is: Chloroflexus aurantiacus
The enzyme appears in selected viruses and cellular organisms
Synonyms
MCT, mesaconyl-CoA C1-C4 CoA transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mesaconyl-CoA C1-C4 CoA transferase
-
SYSTEMATIC NAME
IUBMB Comments
2-methylfumaryl-CoA 1,4-CoA-mutase
The enzyme, purified from the bacterium Chloroflexus aurantiacus, acts as an intramolecular CoA transferase and does not transfer CoA to free mesaconate. It is part of the 3-hydroxypropanoate cycle for carbon assimilation.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methylfumaryl-CoA
3-methylfumaryl-CoA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-methylfumaryl-CoA
3-methylfumaryl-CoA
show the reaction diagram
the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
sodium borohydride has no effect
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
840
2-methylfumaryl-CoA
55°C, pH not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6
at 20°C, 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is part of the 3-hydroxypropanoate cycle for carbon assimilation
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
73000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 47000
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as an N-terminal His10-tagged protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zarzycki, J.; Brecht, V.; Muller, M.; Fuchs, G.
Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus
Proc. Natl. Acad. Sci. USA
106
21317-21322
2009
Chloroflexus aurantiacus (A9WC36)
Manually annotated by BRENDA team