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Information on EC 5.3.99.4 - prostaglandin-I synthase and Organism(s) Rattus norvegicus and UniProt Accession Q62969

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     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.4 prostaglandin-I synthase
IUBMB Comments
A cytochrome P-450 heme-thiolate enzyme. Converts prostaglandin H2 into prostaglandin I2 (prostacyclin).
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q62969
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
pgis, prostacyclin synthase, pgi2 synthase, ptgis, prostacyclin synthetase, pgi2 synthetase, cyp8a1, pgi synthase, prostaglandin i2 synthase, pgi2-s, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
prostacyclin synthase
-
Aortic cytochrom P450
-
-
-
-
PGI2 synthase
PGI2 synthetase
-
-
-
-
prostacyclin synthase
Prostacyclin synthetase
-
-
-
-
prostacyclin/PGI2 synthase
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-
Prostacycline synthetase
-
-
-
-
Prostaglandin I2 synthase
Prostaglandin I2 synthetase
-
-
-
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Synthetase, prostacyclin
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
intramolecular oxidoreduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-9alpha,11alpha-Epidioxy-15-hydroxyprosta-5,13-dienoate 6-isomerase
A cytochrome P-450 heme-thiolate enzyme. Converts prostaglandin H2 into prostaglandin I2 (prostacyclin).
CAS REGISTRY NUMBER
COMMENTARY hide
65802-86-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Prostaglandin H2
Prostaglandin I2
show the reaction diagram
-
-
-
?
prostaglandin H2
6-keto prostaglandin F1-alpha
show the reaction diagram
-
-
-
-
?
Prostaglandin H2
Prostaglandin I2
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Prostaglandin H2
Prostaglandin I2
show the reaction diagram
-
-
-
?
Prostaglandin H2
Prostaglandin I2
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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normal aortic endothelium. Co-distribution of prostaglandin cyclase and cyclooxygenase COX-1, with only minor expression of cyclooxygenase COX-2 in endothelium
Manually annotated by BRENDA team
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ischemic cortex, ipsilateral penumbra area, pyriform cortex, hippocampus, leptomeninges
Manually annotated by BRENDA team
-
normal aortic endothelium. Co-distribution of prostaglandin cyclase and cyclooxygenase COX-1, with only minor expression of cyclooxygenase COX-2 in endothelium
Manually annotated by BRENDA team
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tendon, fascia and arteries leading to the muscle
Manually annotated by BRENDA team
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of cyclic, pregnant, and pseudopregnant rats. Expression of prostacyclin synthase is increases during early pregnancy and decreases gradually from day 5.5 to 6.5. Later during pregnancy, expression of enzyme is maximal on day 12 and decrease to the end of pregnancy. During pseudopregnancy, both prostaglandin D synthase and prostacyclin synthase are increased in a time-dependent manner and are maximal at day 5. Both enzymes are found in luminal as well as glandular epithelial cells and in stroma during late pregnancy
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PTGIS_RAT
501
1
57128
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
determined by SDS-PAGE and Western Blot analysis
56500
determined by SDS-PAGE and Western Blot analysis
56000
-
monomer, SDS-PAGE, Western Blot
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
specific transgenic expression of prostacyclin synthase in mouse kidney results in increased in urinary 6-keto-prostaglandin F1alpha. Transgenic mice demonstrate endotoxemia-related acute kidney injury with low doses of lipopolysaccharide, which do not alter wild-type glomerular filtration rate. They also show increase in renal cAMP content
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GFP-tagged cDNA containing the full-length rat prostacyclin synthase gene is inserted into an adenoviral DNA construct for in vitro gene transfer to murine and human mesenchymal stem cells
specific transgenic expression in mouse kidney using rat KSP-cadherin promoter
expression in alveolar and airway epithelial cells from Mus musculus
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expression in HEK 293 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
angiotensin II (0.1 mM, 24 h incubation) induces PGIS mRNA expression. Pretreatment with losartan (0.01 mM), PD98059 (0.025 mM) or SB203580 (0.01 mM) prevents PGIS protein expression induced by the combination of angiotensin II and interleukin-1beta
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interleukin-1beta (10 ng/ml, 24 h incubation) does not alter PGIS protein expression
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
McLennan, I.S.; MacDonald, R.E.
Prostaglandin synthetase and prostacyclin synthetase in mature rat skeletal muscles: immunohistochemical localisation to arterioles, tendons and connective tissues
J. Anat.
178
243-253
1991
Rattus norvegicus
Manually annotated by BRENDA team
Keith, R.L.; Miller, Y.E.; Hudish, T.M.; Girod, C.E.; Sotto-Santiago, S.; Franklin, W.A.; Nemenoff, R.A.; March, T.H.; Nana-Sinkam, S.P.; Geraci, M.W.
Pulmonary prostacyclin synthase overexpression chemoprevents tobacco smoke lung carcinogenesis in mice
Cancer Res.
64
5897-5904
2004
Rattus norvegicus
Manually annotated by BRENDA team
Fang, Y.C.; Wu, J.S.; Chen, J.J.; Cheung, W.M.; Tseng, P.H.; Tam, K.B.; Shyue, S.K.; Lin, T.N.
Induction of prostacyclin/PGI(2) synthase expression after cerebral ischemia-reperfusion
J. Cereb. Blood Flow Metab.
26
491-501
2006
Rattus norvegicus
Manually annotated by BRENDA team
Wang, W.; Zolty, E.; Falk, S.; Summer, S.; Stearman, R.; Geraci, M.; Schrier, R.
Prostacyclin in endotoxemia-induced acute kidney injury: cyclooxygenase inhibition and renal prostacyclin synthase transgenic mice
Am. J. Physiol. Renal Physiol.
293
F1131-F1136
2007
Rattus norvegicus (Q62969)
Manually annotated by BRENDA team
Kawka, D.W.; Ouellet, M.; Hetu, P.; Singer, I.I.; Riendeau, D.
Double-label expression studies of prostacyclin synthase, thromboxane synthase and COX isoforms in normal aortic endothelium
Biochim. Biophys. Acta
1771
45-54
2007
Canis lupus familiaris, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Kengni, J.H.; St-Louis, I.; Parent, S.; Leblanc, V.; Shooner, C.; Asselin, E.
Regulation of prostaglandin D synthase and prostacyclin synthase in the endometrium of cyclic, pregnant, and pseudopregnant rats and their regulation by sex steroids
J. Endocrinol.
195
301-311
2007
Rattus norvegicus
Manually annotated by BRENDA team
Gillio-Meina, C.; Phang, S.H.; Mather, J.P.; Knight, B.S.; Kennedy, T.G.
Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization
Reproduction
137
537-552
2009
Rattus norvegicus (Q62969)
Manually annotated by BRENDA team
Ishii, M.; Numaguchi, Y.; Okumura, K.; Kubota, R.; Ma, X.; Murakami, R.; Naruse, K.; Murohara, T.
Mesenchymal stem cell-based gene therapy with prostacyclin synthase enhanced neovascularization in hindlimb ischemia
Atherosclerosis
206
109-118
2009
Rattus norvegicus (Q62969)
Manually annotated by BRENDA team
Galan, M.; Miguel, M.; Beltran, A.E.; Rodriguez, C.; Garcia-Redondo, A.B.; Rodriguez-Calvo, R.; Alonso, M.J.; Martinez-Gonzalez, J.; Salaices, M.
Angiotensin II differentially modulates cyclooxygenase-2, microsomal prostaglandin E2 synthase-1 and prostaglandin I2 synthase expression in adventitial fibroblasts exposed to inflammatory stimuli
J. Hypertens.
29
529-536
2011
Rattus norvegicus
Manually annotated by BRENDA team
Tsai, M.J.; Huang, C.T.; Huang, Y.S.; Weng, C.F.; Shyue, S.K.; Huang, M.C.; Liou, D.Y.; Lin, Y.R.; Cheng, C.H.; Kuo, H.S.; Lin, Y.; Lee, M.J.; Huang, W.H.; Huang, W.C.; Cheng, H.
Improving the regenerative potential of olfactory ensheathing cells by overexpressing prostacyclin synthetase and its application in spinal cord repair
J. Biomed. Sci.
24
34
2017
Rattus norvegicus
Manually annotated by BRENDA team