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Information on EC 5.3.99.3 - prostaglandin-E synthase and Organism(s) Bos taurus and UniProt Accession Q66LN0

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EC Tree
     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.99 Other intramolecular oxidoreductases
                5.3.99.3 prostaglandin-E synthase
IUBMB Comments
Brings about the opening of the epidioxy bridge. Requires glutathione.
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This record set is specific for:
Bos taurus
UNIPROT: Q66LN0
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
mpges-1, pges, microsomal prostaglandin e synthase-1, mpges, pge synthase, ptges, prostaglandin e synthase, mpges-2, cpges, mpges1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
microsomal prostaglandin E synthase-2
-
Prostaglandin E synthase
-
cytosolic prostaglandin E synthase
-
endoperoxide isomerase
-
-
-
-
Isomerase, prostaglandin R2 E-
-
-
-
-
microsomal prostaglandin E synthase-1
-
mPGE synthase-2
-
-
MPGES1
-
-
PGE isomerase
-
-
-
-
PGE2 isomerase
-
-
-
-
PGH-PGE isomerase
-
-
-
-
PGH2/PGE2 isomerase
-
-
-
-
prostagandin-E synthetase
-
-
-
-
Prostaglandin E synthase
prostaglandin E synthase-2
-
-
Prostaglandin E2 isomerase
-
-
-
-
Prostaglandin endoperoxide E isomerase
-
-
-
-
Prostaglandin endoperoxide E2 isomerase
-
-
-
-
Prostaglandin endoperoxide-E isomerase
-
-
-
-
Prostaglandin H-E isomerase
-
-
-
-
Prostaglandin H-prostaglandin E isomerase
-
-
-
-
Prostaglandin R-prostaglandin E isomerase
-
-
-
-
Prostaglandin-H2 E-isomerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
intramolecular oxidation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(5Z,13E)-(15S)-9alpha,11alpha-Epidioxy-15-hydroxyprosta-5,13-dienoate E-isomerase
Brings about the opening of the epidioxy bridge. Requires glutathione.
CAS REGISTRY NUMBER
COMMENTARY hide
52227-79-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Prostaglandin H2
Prostaglandin E2
show the reaction diagram
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
show the reaction diagram
-
-
-
-
?
Prostaglandin G1
15-Hydroperoxy-prostaglandin E1
show the reaction diagram
-
at 50% of the activity relative to prostaglandin H
major product
?
prostaglandin G2
15-hydroperoxy-prostaglandin E2
show the reaction diagram
-
at 50% of the activity relative to prostaglandin H
major product
?
Prostaglandin H1
Prostaglandin E1
show the reaction diagram
Prostaglandin H2
Prostaglandin E2
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Bis(3-carboxy-4-nitrophenyl)disulfide
-
-
HgCl2
-
-
iodoacetamide
-
-
iodoacetic acid
-
-
Iodosobenzoic acid
-
-
NEM
-
-
PCMB
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dihydrolipoic acid
-
-
dithiothreitol
glutathione
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
Prostaglandin G1
-
-
0.01
Prostaglandin H1
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.6
-
prostaglandin H1
1.8
-
prostaglandin H2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
enzyme assay
7 - 8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
-
almost inactive below pH 5.5 and above pH 9.5
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
PGE synthase activity is completely lost at 50°C for 5 min
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
prostaglandin E synthases are mostly concentrated in the oviductal epithelial layer and primarily expressed in the ampulla section of the oviduct and to a lesser extent in the isthmus and the isthmic-ampullary junction, the mPGES-2 is mostly expressed in the ipsilateral oviduct
Manually annotated by BRENDA team
faint expression levels
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PGES2_BOVIN
372
0
41738
Swiss-Prot
Mitochondrion (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
SDS-PAGE
16000
SDS-PAGE
23000
SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
pH-range of highest stability, 35°C, 7.5 min, either in presence or absence of glutathione
3129
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
pH 8.0, 0.1 M potassium phosphate buffer, half-life: 30 min
additional information
-
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or cysteine markedly protect from thermal inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
thiol compounds, glutathione, dithiothreitol, 2-mercaptoethanol or Cys markedly protect from thermal inactivation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 mM glutathione, pH 7.0, completely inactivated after a few weeks
-
-70°C, 1 mM glutathione, pH 7.0, stable for at least several months
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
McNamara, D.B.; Hussey, J.L.; Kerstein, M.D.; Rosenson, R.S.; Hyman, A.L.; Kadowitz, P.J.
Modulation of prostacyclin synthetase and unmasking of PGE2 isomerase in bovine coronary arterial microsomes
Biochem. Biophys. Res. Commun.
118
33-39
1984
Bos taurus, Canis lupus familiaris, Ovis aries, Homo sapiens
Manually annotated by BRENDA team
Ogino, N.; Miyamoto, T.; Yamamoto, S.; Hayaishi, O.
Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme
J. Biol. Chem.
252
890-895
1977
Bos taurus
Manually annotated by BRENDA team
Parent, J.; Fortier, M.A.
Expression and contribution of three different isoforms of prostaglandin E synthase in the bovine endometrium
Biol. Reprod.
73
36-44
2005
Bos taurus, Bos taurus (Q3ZBF7), Bos taurus (Q66LN0)
Manually annotated by BRENDA team
Watanabe, K.; Ito, S.; Yamamoto, S.
Studies on membrane-associated prostaglandin E synthase-2 with reference to production of 12L-hydroxy-5,8,10-heptadecatrienoic acid (HHT)
Biochem. Biophys. Res. Commun.
367
782-786
2008
Bos taurus
Manually annotated by BRENDA team
Gauvreau, D.; Moisan, V.; Roy, M.; Fortier, M.A.; Bilodeau, J.F.
Expression of prostaglandin E synthases in the bovine oviduct
Theriogenology
73
103-111
2010
Bos taurus (Q3ZBF7), Bos taurus (Q66LN0), Bos taurus (Q95L14), Bos taurus
Manually annotated by BRENDA team