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Information on EC 5.3.3.6 - methylitaconate DELTA-isomerase
for references in articles please use BRENDA:EC5.3.3.6
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EC Tree 5 Isomerases
5.3 Intramolecular oxidoreductases
5.3.3 Transposing C=C bonds
5.3.3.6 methylitaconate DELTA-isomerase
The expected taxonomic range for this enzyme is: Eubacterium barkeri
showSynonyms
3-methylitaconate-delta-isomerase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Isomerase, methylitaconate
-
-
-
-
Isomerase, methylitaconate DELTA-
-
-
-
-
MeIT isomerase
-
-
-
-
Methylitaconate isomerase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
methylitaconate = 2,3-dimethylmaleate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
isomerization
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
nicotinate degradation III
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SYSTEMATIC NAME
IUBMB Comments
methylitaconate DELTA2-DELTA3-isomerase
-
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CAS REGISTRY NUMBER
COMMENTARY
9059-08-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Methylitaconate
?
-
Substrates: key step in the overall fermentation of nicotinate
Products: -
Products: -
?
Methylitaconate
Dimethylmaleate
-
Substrates: 3-methylitaconate. The enzymatically active enantiomer has (R)-configuration
Products: 2,3-dimethylmaleate
Products: 2,3-dimethylmaleate
?
Methylitaconate
Dimethylmaleate
-
Substrates: formation of dimethylmaleate is favored
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Methylitaconate
?
-
Substrates: key step in the overall fermentation of nicotinate
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
not detectable in cells grown on glucose
brenda
Highest Expressing Human Cell Lines
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, protein concentration 1 mg/ml, little loss of activity after 60 days
-
-80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kung, H.F.; Tsai, L.
Nicotinic acid metabolism. VII. Mechanism of action of Clostridial alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase
J. Biol. Chem.
246
6436-6443
1971
Eubacterium barkeri
brenda
Hartrampf, G.; Buckel, W.
On the steric course of the adenosylcobalamin-dependent 2-methyleneglutarate mutase reaction in Clostridium barkeri
Eur. J. Biochem.
156
301-304
1986
Eubacterium barkeri
brenda
Kung, H.F.; Stadtman, T.C.
Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase
J. Biol. Chem.
246
3378-3388
1971
Eubacterium barkeri
brenda
Alhapel, A.; Darley, D.J.; Wagener, N.; Eckel, E.; Elsner, N.; Pierik, A.J.
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri
Proc. Natl. Acad. Sci. USA
103
12341-12346
2006
Eubacterium barkeri
brenda
Velarde, M.; Macieira, S.; Hilberg, M.; Brker, G.; Tu, S.-M.; Golding, B.T.; Pierik, A.J.; Buckel, W.; Messerschmidt, A.
Crystal structure and putative mechanism of 3-methylitaconate-DELTA-isomerase from Eubacterium barkeri
J. Mol. Biol.
391
609-620
2009
Eubacterium barkeri (Q0QLE6), Eubacterium barkeri
brenda
EXTERNAL LINKS (specific for EC-Number 5.3.3.6)
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