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Information on EC 5.3.3.5 - cholestenol DELTA-isomerase and Organism(s) Rattus norvegicus and UniProt Accession Q9JJ46

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EC Tree
     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.3 Transposing C=C bonds
                5.3.3.5 cholestenol DELTA-isomerase
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9JJ46 not found.
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
emopamil binding protein, emopamil-binding protein, delta8-delta7 sterol isomerase, c-8,7 sterol isomerase, erg2p, delta8-delta7 isomerase, d8d7i, 8,7si, cholestenol delta-isomerase, d8-d7 sterol isomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sterol DELTA8 isomerase
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Cholestenol delta-isomerase
-
-
-
-
D8-D7 sterol isomerase
-
-
-
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DELTA8-Cholestenol-DELTA7-cholestenol isomerase
-
-
-
-
Delta8-delta7 sterol isomerase
-
-
-
-
DELTA8-SI
-
-
Emopamil binding protein
-
-
-
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Isomerase, cholestenol DELTA-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
intramolecular oxidoreduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
DELTA7-Cholestenol DELTA7-DELTA8-isomerase
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CAS REGISTRY NUMBER
COMMENTARY hide
37318-47-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5alpha-cholest-7-en-3beta-ol
?
show the reaction diagram
enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs
-
?
5alpha-Cholest-7-en-3beta-ol
5alpha-Cholest-8-en-3beta-ol
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5alpha-cholest-7-en-3beta-ol
?
show the reaction diagram
enzyme of the cholesterol biosynthetic pathway, mutations are associated with Conradi-Huenermann syndrome. The enzyme may be involved in liver function, since the mRNA is only detected in hepatocytes not in connective tissue or blood vessels. The expression of the SI gene is regulated transcriptionally in vitro and in vivo by widely known regulatory agents ranging from tamoxifen, an oestrogen receptor antagonist and SI-specific inhibitor, to cholesterol-lowering drugs
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one
IC50: 0.0042 mM
trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine
IC50: 0.0112 mM
2-[4-[(1Z)-1,2-diphenylbut-1-en-1-yl]phenoxy]-N,N-dimethylethanamine
-
noncompetitive, IC50: 0.00025 mM
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00021
tamoxifen
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0042
3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one
Rattus norvegicus
IC50: 0.0042 mM
0.0112
trans-2-[4-(1,2-diphenylbuten-1-yl)phenoxy]-N,N-dimethylethylamine
Rattus norvegicus
IC50: 0.0112 mM
0.00025
2-[4-[(1Z)-1,2-diphenylbut-1-en-1-yl]phenoxy]-N,N-dimethylethanamine
Rattus norvegicus
-
noncompetitive, IC50: 0.00025 mM
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EBP_RAT
230
5
26738
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26737
x * 26737, calculation from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 26737, calculation from nucleotide sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, W.H.; Kammereck, R.; Lutsky, B.N.; McCloskey, J.A.; Schroepfer, G.J.Jr.
Studies on the mechanism of the enzymatic conversion of DELTA8-cholesten-3beta-ol to DELTA7-cholesten-3beta-ol
J. Biol. Chem.
244
2033-2040
1969
Rattus norvegicus
Manually annotated by BRENDA team
Wilton, D.C.; Rahimtula, A.D.; Akhtar, M.
The reversibility of the DELTA8-cholestenol-DELTA7-cholestenol isomerase reaction in cholesterol biosynthesis
Biochem. J.
114
71-73
1969
Rattus norvegicus
Manually annotated by BRENDA team
Scala, A.; Galli-Kienle, M.; Anastasia, M.; Galli, G.
The reversibility of the isomerization of the DELTA8 to DELTA7 bond in cholesterol biosynthesis
Eur. J. Biochem.
48
263-269
1974
Rattus norvegicus
Manually annotated by BRENDA team
Bae, S.H.; Seong, J.; Paik, Y.K.
Cholesterol biosynthesis from lanosterol: molecular cloning, chromosomal localization, functional expression and liver-specific gene regulation of rat sterol DELTA8-isomerase, a cholesterogenic enzyme with multiple functions
Biochem. J.
353
689-699
2001
Rattus norvegicus (Q9JJ46)
Manually annotated by BRENDA team
Cho, S.Y.; Kim, J.H.; Paik, Y.K.
Cholesterol biosynthesis from lanosterol: differential inhibition of sterol DELTA8-isomerase and other lanosterol-converting enzymes by tamoxifen
Mol. Cells
8
233-239
1998
Rattus norvegicus
Manually annotated by BRENDA team