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Information on EC 5.3.3.4 - muconolactone DELTA-isomerase
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EC Tree
5.3.3.4 muconolactone DELTA-isomeraseSpecify your search results
Word Map
- 5.3.3.4
- putida
-
cycloisomerase
-
beta-ketoadipate
- 3-oxoadipate
- isomerases
- catechols
-
rhodococcus
- muconate
- acinetobacter
-
lactonizing
- calcoaceticus
- enol-lactone
-
chloromuconate
- cis-dienelactone
-
decameric
-
dechlorination
- cis,cis-muconate
- opacus
-
5.5.1.1
- 3-chlorocatechol
- eutrophus
-
alcaligenes
-
1,2-dioxygenase
-
dehalogenase
- 5-chloromuconolactone
-
catbc
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
muconolactone isomerase, miase, methylmuconolactone isomerase, muconolactone delta-isomerase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CATC
EC 3.1.1.16
-
-
formerly, part transferred
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Isomerase, muconolactoneDELTA-
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-
-
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Methylmuconolactone isomerase
MI
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-
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MIase
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-
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MLI
Muconolactone DELTA-isomerase
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-
-
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Muconolactone isomerase
Methylmuconolactone isomerase
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-
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Muconolactone isomerase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(+)-muconolactone = (4,5-dihydro-5-oxofuran-2-yl)-acetate
stereochemical course
-
(+)-muconolactone = (4,5-dihydro-5-oxofuran-2-yl)-acetate
stereochemical course
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(+)-muconolactone = (4,5-dihydro-5-oxofuran-2-yl)-acetate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
isomerization
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
5-oxo-4,5-dihydrofuran-2-acetate Delta3-Delta2-isomerase
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CAS REGISTRY NUMBER
COMMENTARY
37318-46-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
(2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
(S)-5-oxo-2,5-dihydrofuran-2-acetate
5-oxo-4,5-dihydrofuran-2-acetate
-
-
-
?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5R/4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
2-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5S)-(+)-5-chloromuconolactone
(2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5S)-(+)-5-chloromuconolactone
(2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
(4R,5S)-(+)-5-chloromuconolactone
(2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5S)-(+)-5-chloromuconolactone
(2S)-chloro(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
(2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
-
?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
(2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
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preferred substrate over the natural substrate (4S)-muconolactone
-
?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
(2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
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-
-
-
?
(4R,5S)-(+/-)-5-chloro-3-methylmuconolactone
(2S)-chloro(3-methyl-5-oxo-4,5-dihydrofuran-2-yl)acetic acid
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preferred substrate over the natural substrate (4S)-muconolactone
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?
(4S)-muconolactone
5-oxo-3,4-dihydrofuran-2-acetate
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-
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
-
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
(4S)-(+)-muconolactone
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
(4S)-(+)-muconolactone
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
(4S)-(+)-muconolactone
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
(4S)-(+)-muconolactone
-
?
muconolactone
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
Pseudomonas putida A.3.12. / ATCC 12633
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-
-
?
additional information
?
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-
enzyme of beta-ketoadipate pathway
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-
?
additional information
?
-
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formation of protoanemonin from 2-chloro-cis,cis-muconate by the combined action of muconate cycloisomerase and muconolactone isomerase
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?
additional information
?
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formation of protoanemonin from 2-chloro-cis,cis-muconate by the combined action of muconate cycloisomerase and muconolactone isomerase
-
?
additional information
?
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enzyme is required for the dissimilation of benzoate to beta-ketoadipate
-
-
?
additional information
?
-
Pseudomonas putida A.3.12. / ATCC 12633
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enzyme of beta-ketoadipate pathway
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-5-oxo-2,5-dihydrofuran-2-acetate
5-oxo-4,5-dihydrofuran-2-acetate
-
-
-
?
additional information
?
-
-
enzyme of beta-ketoadipate pathway
-
-
?
additional information
?
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formation of protoanemonin from 2-chloro-cis,cis-muconate by the combined action of muconate cycloisomerase and muconolactone isomerase
-
?
additional information
?
-
-
formation of protoanemonin from 2-chloro-cis,cis-muconate by the combined action of muconate cycloisomerase and muconolactone isomerase
-
?
additional information
?
-
-
enzyme is required for the dissimilation of benzoate to beta-ketoadipate
-
-
?
additional information
?
-
Pseudomonas putida A.3.12. / ATCC 12633
-
enzyme of beta-ketoadipate pathway
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(4S,5S)-(+/-)-5-chloro-3-methylmuconolactone
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isomerization of (4R,5R)-(+/-)-5-chloro-3-methylmuconolactone
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
9
-
5R/4R,5S)-(+/-)-5-chloro-3-methylmuconolactone, more active in potassium sodium phosphate buffer than in Tris/HCl or borate buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 9.5
-
pH 7: about 80% of maximal activity, pH 9.5: about 70% of maximal activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
the mml gene cluster consists of 10 genes encoding enzymes involved in the metabolism of 4-methylmuconolactone
Show AA Sequence and Transmembrane Helices (3947 entries)
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Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100000
11000
-
10 * 11000, crystallographic data, decamer with a 5fold symmetry axis
11300
12000
93000
additional information
-
partial amino acid sequence of cyanogen bromide fragments of the enzyme
11300
-
or dodecamer, 10 * or 12 * 11300, SDS-PAGE. Oligomer may be composed of two stacked discs of pentamers or hexamers
11300
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or decamer, 12 * or 10 * 11300, SDS-PAGE. Oligomer may be composed of two stacked discs of pentamers or hexamers
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decamer
dodecamer
-
or decamer, 12 * or 10 * 11300, SDS-PAGE. Oligomer may be composed of two stacked discs of pentamers or hexamers
octamer
decamer
-
10 * 11000, crystallographic data, decamer with a 5fold symmetry axis
decamer
-
or dodecamer, 10 * or 12 * 11300, SDS-PAGE. Oligomer may be composed of two stacked discs of pentamers or hexamers
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
90
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GENERAL STABILITY
ORGANISM
UNIPROT