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Information on EC 5.3.1.13 - arabinose-5-phosphate isomerase and Organism(s) Pseudomonas aeruginosa and UniProt Accession Q9HVW0

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IUBMB Comments
The enzyme is involved in the pathway for synthesis of 3-deoxy-D-manno-octulosonate (Kdo), a component of bacterial lipopolysaccharides and plant call walls.
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This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: Q9HVW0
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
d-arabinose 5-phosphate isomerase, arabinose 5-phosphate isomerase, arabinose-5-phosphate isomerase, k-api, d-arabinose-5-phosphate isomerase, c3406 protein, a5p isomerase, d-arabinose-5p isomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Arabinose phosphate isomerase
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Arabinose phosphate isomerase, arabinose phosphate
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D-Arabinose 5-phosphate isomerase
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D-arabinose-5-phosphate ketol-isomerase
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-
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D-arabinose-5P isomerase
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Isomerase, arabinose phosphate
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-
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Phosphoarabinoisomerase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
intramolecular oxidoreduction
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
D-arabinose-5-phosphate aldose-ketose-isomerase
The enzyme is involved in the pathway for synthesis of 3-deoxy-D-manno-octulosonate (Kdo), a component of bacterial lipopolysaccharides and plant call walls.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-86-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-deoxy-D-threo-pentofuranose 5-phosphate
3-deoxy-D-glycero-pentulose 5-phosphate
show the reaction diagram
-
the catalytic efficiency is about 1000times lower than for the natural substrate D-arabinose 5-phosphate
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-
?
4-deoxy-D-threo-pentofuranose 5-phosphate
4-deoxy-D-glycero-pentulose 5-phosphate
show the reaction diagram
-
the catalytic efficiency is comparable with the natural substrate D-arabinose 5-phosphate
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?
D-Arabinose 5-phosphate
D-Ribulose 5-phosphate
show the reaction diagram
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-
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r
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-Arabinose 5-phosphate
D-Ribulose 5-phosphate
show the reaction diagram
-
-
-
-
r
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.092
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mutant enzyme K56A, in Tris·HCl, (pH 8.5, 50 mM), at 37°C
29.5
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recombinant wild type enzyme, in Tris·HCl, (pH 8.5, 50 mM), at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme is involved in the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid and thus of lipopolysaccharide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
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x * 34000, SDS-PAGE
34067
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x * 34067, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H190A
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inactive
H85A
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inactive
K56A
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the mutant shows 0.31% residual activity compared to the wild type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Airoldi, C.; Sommaruga, S.; Merlo, S.; Sperandeo, P.; Cipolla, L.; Polissi, A.; Nicotra, F.
Targeting bacterial membranes: identification of Pseudomonas aeruginosa D-arabinose-5P isomerase and NMR characterisation of its substrate recognition and binding properties
ChemBioChem
12
719-727
2011
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Gabrielli, L.; Merlo, S.; Airoldi, C.; Sperandeo, P.; Gianera, S.; Polissi, A.; Nicotra, F.; Holler, T.P.; Woodard, R.W.; Cipolla, L.
Arabinose 5-phosphate isomerase as a target for antibacterial design studies with substrate analogues and inhibitors
Bioorg. Med. Chem.
22
2576-2583
2014
Pseudomonas aeruginosa (Q9HVW0), Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644 (Q9HVW0)
Manually annotated by BRENDA team