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Information on EC 5.2.1.5 - linoleate isomerase
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EC Tree
5.2.1.5 linoleate isomeraseSpecify your search results
Word Map
- 5.2.1.5
- lactobacillus
- propionibacterium
- plantarum
- acnes
-
rumen
- fibrisolvens
- butyrivibrio
-
probiotic
- synthesis
-
biohydrogenation
-
clnas
-
linolenic
- acidophilus
-
anti-carcinogenic
- acetoacetate
-
bifidobacteria
-
anti-atherogenic
-
oleaginous
- biotechnology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
linoleate isomerase, linoleic acid isomerase, la isomerase, la-i, linoleic acid delta12-cis,delta11-trans-isomerase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C12 isomerase
FG02668.1
Isomerase, linoleate
-
-
-
-
IV39_GL000089
LA isomerase
LA-I
LAI
LAI12
Linoleate DELTA12-cis,DELTA11-trans isomerase
-
-
-
-
linoleic acid DELTA12-cis,DELTA11-trans-isomerase
Linoleic acid isomerase
PAI
Linoleic acid isomerase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
9-cis,12-cis-octadecadienoate = 9-cis,11-trans-octadecadienoate
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-
-
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9-cis,12-cis-octadecadienoate = 9-cis,11-trans-octadecadienoate
mechanism of the biocatalysis of linoleic acid to conjugated linoleic acid, which are a group of geometrical and positional isomers of linoleic acid with conjugated double bonds
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9-cis,12-cis-octadecadienoate = 9-cis,11-trans-octadecadienoate
mechanism of the biocatalysis of linoleic acid to conjugated linoleic acid, which are a group of geometrical and positional isomers of linoleic acid with conjugated double bonds
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9-cis,12-cis-octadecadienoate = 9-cis,11-trans-octadecadienoate
mechanism of the biocatalysis of linoleic acid to conjugated linoleic acid, which are a group of geometrical and positional isomers of linoleic acid with conjugated double bonds
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cis-trans-isomerization
cis-trans-isomerization
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
linoleate DELTA12-cis-DELTA11-trans-isomerase
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CAS REGISTRY NUMBER
COMMENTARY
37318-41-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid
?
-
i.e. stearidonic acid
-
-
?
6-cis,9-cis,12-cis-octadecatrienoic acid
?
-
i.e. alpha-linolenic acid
-
-
?
9-cis,12-cis,15-cis-octadecatrienoic acid
?
-
i.e. gamma-linolenic acid
-
-
?
9-cis,12-cis-Octadecadienoate
?
-
initial reaction of the biohydrogenation pathway
-
-
?
cis-9,cis-12,cis-15-octadecatrienoic acid
cis-9,trans-11,cis-15-octadecatrienoic acid
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-
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid
(9Z,11E)-octadeca-9,11-dienoic acid
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-
i.e. rumenic acid
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?
(9Z,12Z)-octadeca-9,12-dienoic acid
(9Z,11E)-octadeca-9,11-dienoic acid
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-
i.e. rumenic acid
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid
(9Z,11E)-octadeca-9,11-dienoic acid
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-
i.e. rumenic acid
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid
(9Z,11E)-octadeca-9,11-dienoic acid
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-
i.e. rumenic acid
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid
(9Z,11E)-octadeca-9,11-dienoic acid
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-
i.e. rumenic acid
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?
9-cis,11-trans-linoleic acid
9-trans,11-trans-linoleic acid
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formation only under aerobic conditions
-
-
?
9-cis,11-trans-linoleic acid
9-trans,11-trans-linoleic acid
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-
-
?
9-cis,11-trans-linoleic acid
9-trans,11-trans-linoleic acid
Lactiplantibacillus plantarum ZS20580
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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higher activity under aerobic than under anaerobic conditions
conjugated linoleic acid isomer
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
-
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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the enzyme shows high specificity for the production of the cis-9, trans-11 conjugated linoleic acid isomer
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-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
Limosilactobacillus reuteri ATCC 55739
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the enzyme shows high specificity for the production of the cis-9, trans-11 conjugated linoleic acid isomer
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-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid + 9-trans,11-trans-linoleic acid
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-
-
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?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid + 9-trans,11-trans-linoleic acid
Lactiplantibacillus plantarum AKU1009a
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-
-
-
?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
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-
-
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?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
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-
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?
9-cis,12-cis-Octadecadienoate
9-cis,11-trans-Octadecadienoate
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-
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?
9-cis,12-cis-Octadecadienoate
9-cis,11-trans-Octadecadienoate
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r
-
?
9-cis,12-cis-Octadecadienoate
9-cis,11-trans-Octadecadienoate
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9,12-octadienoic acid
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?
additional information
?
-
-
substrate specificity, overview, the enzyme is specific for substrates containing cis double bonds at the c9 and c12 positions of C18 polyunsaturated fatty acids, a free carboxyl group is absolutely necessary for isomerization
-
-
?
additional information
?
-
enzyme shows oleate hydratase activity, reaction of EC 4.2.1.53, but does not display linoleate isomerase activity
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
9-cis,11-trans-linoleic acid
9-trans,11-trans-linoleic acid
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formation only under aerobic conditions
-
-
?
9-cis,12-cis-Octadecadienoate
?
-
initial reaction of the biohydrogenation pathway
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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higher activity under aerobic than under anaerobic conditions
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-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-cis,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
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-
-
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?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
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-
-
-
?
9-cis,12-cis-linoleic acid
9-trans,11-trans-linoleic acid
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no external cofactors are required for catalysis
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
metal ions such as Co2+, Ni2+, Fe3+, Cd3+, and Mn2+ restore conjugated linoleic acid productivity after inhibition with o-phenanthroline up to 56% of the original activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10,13-Octadecadienoic acid
-
inhibits isomerization of 9,12-octadienoic acid
11,14-Octadecadienoic acid
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inhibits isomerization of 9,12-octadienoic acid
12,15-Octadecadienoic acid
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inhibits isomerization of 9,12-octadienoic acid
4,7-Octadecadienoic acid
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inhibits isomerization of 2,5-octadecadienoic acid and 9,12-octadecadienic acid
5,8-Octadecadienoic acid
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inhibits isomerization of 2,5-octadecadienoic acid and 9,12-octadecadienic acid
7,10-Octadecadienoic acid
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inhibits isomerization of 9,12-octadienoic acid
8,11-Octadecadienoic acid
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inhibits isomerization of 9,12-octadienoic acid
9-cis,12-cis-linoleic acid methyl ester
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i.e. methyl linoleate, 31% inhibition at 0.02 mM
9-cis,12-cis-octadecadien-1-ol
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i.e. linoleyl alcohol, 19% inhibition at 0.02 mM
cis,cis,3,6-Octadecadienoic acid
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inhibits isomerization of 2,5-octadecadienoic acid and 9,12-octadecadienic acid
cis-11,cis-14-Eicosadienic acid
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inhibits isomerization of 9-cis,12-cis-octadecadienoate
cis-6,cis-9-Octadecadienic acid
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inhibits isomerization of 9-cis,12-cis-octadecadienoate
cis-9,trans-12-Octadecadienoic acid
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
cis-9-Octadecen-12-ynoic acid
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
gamma-linolenic acid
-
substrate inhibition at concentrations above 0.015 mM
Linoleamide
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
Linoleyl alcohol
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
linoleyl amine
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
Linoleyl hydroxamate
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
Linoleyl methyl ether
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inhibits isomerization of 9-cis,12-cis-octadecadienoate
Linoleyl oxime
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
methyl oleate
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
oleyl alcohol
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
oleyl amine
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
stearidonic acid
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substrate inhibition at concentrations above 0.120 mM
trans-11-Octadecen-9-ynoic acid
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
trans-3,cis-5-Octadecadienoic acid
-
inhibits isomerization of 2,5-octadecadienoic acid
trans-9,cis-12-Octadecadienoic acid
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
trans-9,trans-12-Octadecadienoic acid
-
inhibits isomerization of 9-cis,12-cis-octadecadienoate
additional information
-
no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA
-
linoleic acid
-
substrate inhibition at concentrations above 0.02 mM, some linoleic acid derivatives also inhibited the isomerase
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
the addition of 5 mM NADH plus FAD to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 101%
K2MoO4
-
422% activity at 5 mM, in the presence of o-phenanthroline
K2WO4
-
149% activity at 5 mM, in the presence of o-phenanthroline
Na2MnO4
-
427% activity at 5 mM, in the presence of o-phenanthroline
Na2WO4
-
157% activity at 5 mM, in the presence of o-phenanthroline
Na3VO4
-
264% activity at 5 mM, in the presence of o-phenanthroline
NADH
-
the addition of 5 mM NADH plus FAD to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 101%
NADPH
-
the addition of 5 mM NADPH to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 98%
NaVO3
-
209% activity at 5 mM, in the presence of o-phenanthroline
additional information
-
no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Colitis
Linoleate Isomerase Complex Contributes to Metabolism and Remission of DSS-Induced Colitis in Mice of Lactobacillus plantarum ZS2058.
Insulin Resistance
Importance of Adherence to Personalized Diet Intervention in Obesity Related Metabolic Improvement in Overweight and Obese Korean Adults.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0034
6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid
-
pH 7.5, 22°C
additional information
additional information
-
kinetics for biocatalysis of linoleic acid to conjugated linoleic acid
-
additional information
additional information
-
kinetics for biocatalysis of linoleic acid to conjugated linoleic acid
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
353.5
-
after 93.3fold purification, at pH 6.5, 30°C
additional information
-
higher enzyme activity and more tolerant to linoleic acid than other strains examined
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
