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Information on EC 5.1.3.20 - ADP-glyceromanno-heptose 6-epimerase

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IUBMB Comments
Requires NAD+.
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This record set is specific for:
UNIPROT: B5Z7L9
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
hp0859, adp-l-glycero-d-mannoheptose-6-epimerase, adp-l-glycero-d-manno-heptose 6-epimerase, adp-l-glycero-d-manno-heptose-6-epimerase, adp-l-glycero-d-mannoheptose 6-epimerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADP-L-glycero-D-manno-heptose-6-epimerase
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Adenosine diphosphoglyceromannoheptose 6-epimerase
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ADP-glyceromanno-heptose 6-epimerase
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ADP-L-glycero-D-manno-heptose-6-epimerase
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ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD
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ADP-L-glycero-D-mannoheptose 6-epimerase
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ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme)
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ADP-L-glycero-D-mannoheptose-6-epimerase
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ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD)
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Epimerase, adenosine diphosphoglyceromannoheptose 6-
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Epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced)
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Epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6)
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Genbank AE000440-derived protein GI 1790049
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GenBank AE000684-derived protein GI 2983012
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ADP-L-glycero-D-manno-heptose 6-epimerase
Requires NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
130527-81-0
epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced) /ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD) /genBank AE000440-derived protein GI 1790049
167617-20-1
epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6), ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme)
204790-62-5
ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD /GenBank AE000684-derived protein GI 2983012
85030-75-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
preferred cofactor compared to NADP+, binds at the enzyme active site, binding site structure, overview
NADP+
NAD+ is the preferred cofactor
additional information
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
sequence calculation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene HP0859 or rfaD
UniProt
Manually annotated by BRENDA team
gene HP0859 or rfaD
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
a HP0859 knockout mutant shows a severe loss of lipopolysaccharide structure and a significant reduction of adhesion levels in an infection model to human stomach gastric adenocarcinoma AGS cells, if compared with the wild-type strain
metabolism
last enzyme in the pathway that produces L-glycero-D-manno-heptose starting from sedoheptulose-7-phosphate
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
B5Z7L9_HELPG
Helicobacter pylori (strain G27)
330
0
37437
TrEMBL
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer
structure modeling of the pentameric enzyme including five protein monomers and 5 NAD+ molecules, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme, vapor diffusion technique, mixing of 20 mg/ml protein in 30 mM Tris, pH 7.5, 150 mM NaCl, with precipitant solution containing 0.2 M ammonium sulfate, 0.1 M tri sodium citrate, pH 5.6, 15% w/v PEG 4000, and 5% glycerol, 20°C, X-ray diffraction structure determination and analysis at 2.55 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gell filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene HP0859, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shaik, M.M.; Zanotti, G.; Cendron, L.
The crystal structure of ADP-L-glycero-D-manno-heptose-6-epimerase (HP0859) from Helicobacter pylori
Biochim. Biophys. Acta
1814
1641-1647
2011
Helicobacter pylori (B5Z7L9), Helicobacter pylori, Helicobacter pylori G27 (B5Z7L9)
Manually annotated by BRENDA team