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Information on EC 5.1.1.5 - lysine racemase and Organism(s) Oenococcus oeni and UniProt Accession Q04HB7

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.5 lysine racemase
IUBMB Comments
The enzyme is involved in a lysine catabolic pathway.
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This record set is specific for:
Oenococcus oeni
UNIPROT: Q04HB7
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Word Map
The taxonomic range for the selected organisms is: Oenococcus oeni
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
lysine racemase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Racemase, lysine
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
lysine racemase
The enzyme is involved in a lysine catabolic pathway.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-10-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-lysine
L-lysine
show the reaction diagram
-
-
-
r
D-ornithine
L-ornithine
show the reaction diagram
-
-
-
r
L-lysine
D-lysine
show the reaction diagram
L-Ornithine
D-Ornithine
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lysine
D-lysine
show the reaction diagram
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
dependent on
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 31
D-Lysine
22
D-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
2 - 27
L-lysine
27
L-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 2.83
D-Lysine
1.83
D-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
0.16 - 3.5
L-lysine
1.83
L-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.28
D-Lysine
0.08
D-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
0.006 - 0.32
L-lysine
0.07
L-ornithine
pH 8.0, 30°C, recombinant wild-type enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene OEOE0162
UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T224I
site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme
T224I/W355Y
site-directed mutagenesis, the double mutant shows significantly decreased activity compared to the wild-type enzyme
W355Y
site-directed mutagenesis, the mutant shows significantly decreased activity compared to the wild-type enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene OEOE0162, DNA and amino acid sequence determination and analysis, expression of the enzyme in Escherichia coli strain BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kato, S.; Hemmi, H.; Yoshimura, T.
Lysine racemase from a lactic acid bacterium, Oenococcus oeni: structural basis of substrate specificity
J. Biochem.
152
505-508
2012
Oenococcus oeni (Q04HB7), Oenococcus oeni
Manually annotated by BRENDA team