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Information on EC 5.1.1.10 - amino-acid racemase and Organism(s) Pseudomonas taetrolens and UniProt Accession I0J1I6

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.10 amino-acid racemase
IUBMB Comments
A pyridoxal-phosphate protein.
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This record set is specific for:
Pseudomonas taetrolens
UNIPROT: I0J1I6
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas taetrolens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
amino acid racemase, acl racemase, broad-spectrum amino acid racemase, broad specificity amino acid racemase, amino-acid racemase, broad-specificity amino acid racemase, broad substrate specificity amino acid racemase, gknsaar, n-succinylamino acid racemase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino acid racemase
-
L-Amino acid racemase
-
-
-
-
Racemase, amino acid
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
amino-acid racemase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-61-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Ala
L-Ala
show the reaction diagram
12% activity, compared to D-Lys
-
-
?
D-Arg
L-Arg
show the reaction diagram
95% activity, compared to D-Lys
-
-
?
D-ethionine
L-ethionine
show the reaction diagram
11% activity, compared to D-Lys
-
-
?
D-homoarginine
L-homoarginine
show the reaction diagram
6.2% activity, compared to D-Lys
-
-
?
D-Lys
L-Lys
show the reaction diagram
100% activity
-
-
?
D-Met
L-Met
show the reaction diagram
12% activity, compared to D-Lys
-
-
?
D-norvaline
L-norvaline
show the reaction diagram
6.5% activity, compared to D-Lys
-
-
?
D-ornithine
L-ornithine
show the reaction diagram
42% activity, compared to D-Lys
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1120
recombinant protein
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
optimal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
maximum activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BSR_PSETA
409
0
43976
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41500
calculated from cDNA
84000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 41500 Da
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
N-terminal signal sequence is cleaved off
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matsui, D.; Oikawa, T.; Arakawa, N.; Osumi, S.; Lausberg, F.; Staebler, N.; Freudl, R.; Eggeling, L.
A periplasmic, pyridoxal-5-phosphate-dependent amino acid racemase in Pseudomonas taetrolens
Appl. Microbiol. Biotechnol.
83
1045-1054
2009
Pseudomonas taetrolens (I0J1I6), Pseudomonas taetrolens
Manually annotated by BRENDA team