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EC Tree
The taxonomic range for the selected organisms is: Peribacillus psychrosaccharolyticus The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
alanine racemase,
alr-2 , d-alanine racemase, alrbax, mbalr2, alrtt, l-alanine racemase, alraba, cdalr, alr2 racemase,
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L-Alanine racemase
-
-
-
-
L-Alanine:D-alanine racemase
-
-
-
-
Racemase, alanine
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-
-
-
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alanine racemase
A pyridoxal-phosphate protein.
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pyridoxal 5'-phosphate
Km: 0.005 mM, at 30°C
pyridoxal 5'-phosphate
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Km at 30°C is 0.005 mM. Maximal activity is obtained in presence of more than 0.125 mM pyridoxal 5'-phosphate. The decrease in activity at incubation temperatures over 40°C is consistent with the decrease in the amount of bound pyridoxal 5'-phosphate
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35
in absence of pyridoxal 5'-phosphate
60
in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM
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0 - 60
about 30% of maximal activity at 0°C and at 60°C, in absence of pyridoxal 5'-phosphate
30 - 50
30°C, 45% of maximal activity, 50°C: about 50% of maximal activity, in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM
40 - 70
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40°C: about 60% of maximal activity, 70°C: about 50% of maximal activity
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SwissProt
brenda
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ALR_PERPY
383
0
42519
Swiss-Prot
-
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42500
2 * 42500, SDS-PAGE
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dimer
2 * 42500, SDS-PAGE
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35
pH 8.3, 1 h, the purified is extremely labile over 35°C
50
pH 8.3, 1 h, stabilized up to 50°C in presence of excess amounts of pyridoxal 5'-phosphate, 0.4 mM, complete inactivation in absence of pyridoxal 5'-phosphate
40
-
the decrease in the enzyme activity at incubation temperatures over 40°C is consistent with the decrease in the amount of bound pyridoxal 5'-phosphate, no effect on stability at lower temperatures, 0-30°C
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pyridoxal 5'-phosphate present in the solvent suppresses the enzyme's unfolding
-
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expression in Escherichia coli SOLR with a plasmid yYOK3
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Okubo, Y.; Yokoigawa, K.; Esaki, N.; Soda, K.; Kawai, H.
Characterization of psychrophilic alanine racemase from Bacillus psychrosaccharolyticus
Biochem. Biophys. Res. Commun.
256
333-340
1999
Peribacillus psychrosaccharolyticus (Q9S5V6), Peribacillus psychrosaccharolyticus
brenda
Okubo, Y.; Yokoigawa, K.; Esaki, N.; Soda, K.; Misono, H.
High catalytic activity of alanine racemase from psychrophilic Bacillus psychrosaccharolyticus at high temperatures in the presence of pyridoxal 5'-phosphate
FEMS Microbiol. Lett.
192
169-173
2000
Peribacillus psychrosaccharolyticus
brenda