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Information on EC 5.1.1.1 - alanine racemase and Organism(s) Schizosaccharomyces pombe and UniProt Accession O59828

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EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.1 alanine racemase
IUBMB Comments
A pyridoxal-phosphate protein.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: O59828
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Word Map
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
alanine racemase, alr-2, d-alanine racemase, alrbax, mbalr2, alrtt, l-alanine racemase, alraba, cdalr, alr2 racemase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-Alanine racemase
-
-
-
-
L-Alanine:D-alanine racemase
-
-
-
-
Racemase, alanine
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
racemization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
alanine racemase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-06-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-2-Aminobutyrate
D-2-Aminobutyrate
show the reaction diagram
0.37% of the activity with L-Ala
-
r
L-Ser
D-Ser
show the reaction diagram
3.7% of the activity with L-Ala
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
coenzyme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium borohydride
complete inactivation after dialysis against
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.4
D-Ala
37°C, pH 9.0
5
L-Ala
37°C, pH 9.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
D-Ala as substrate
9.5
L-Ala as substrate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 44000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. Heterologous expression renders Saccharomyces cerevisiae capable of utilization of D-Ala as a nitrogen source but also relieves the yeast from the toxicity of D-Ala
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Uo, T.; Yoshimura, T.; Tanaka, N.; Takegawa, K.; Esaki, N.
Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase
J. Bacteriol.
183
2226-2233
2001
Schizosaccharomyces pombe (O59828), Schizosaccharomyces pombe
Manually annotated by BRENDA team