Information on EC - phenylacetaldoxime dehydratase

for references in articles please use BRENDA:EC4.99.1.7
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IUBMB Comments
The enzyme from Bacillus sp. OxB-1 contains protoheme IX, the iron of which must be in the form iron(II) for activity. (Z)-Phenylacetaldoxime binds to ferric heme (the iron(III) form) via the oxygen atom whereas it binds to the active ferrous form via the nitrogen atom. In this way, the oxidation state of the heme controls the coordination stucture of the substrate---heme complex, which regulates enzyme activity . The enzyme is active towards several (Z)-arylacetaldoximes and (E/Z)-alkylaldoximes as well as towards arylalkylaldoximes such as 3-phenylpropionaldoxime and 4-phenylbutyraldoxime. However, it is inactive with phenylacetaldoximes that have a substituent group at an alpha-site of an oxime group, for example, with (E/Z)-2-phenylpropionaldoxime and (E/Z)-mandelaldoxime. The activity of the enzyme is inhibited completely by the heavy-metal cations Cu+, Cu2+, Ag+ and Hg+ whereas Fe2+ and Sn2+ have an activatory effect.
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
aldoxime dehydratase, OXD, OxdB, OxdFG, OxdRE, phenylacetaldoxime dehydratase, UP10_21065, more
(Z)-phenylacetaldehyde oxime = phenylacetonitrile + H2O
show the reaction diagram
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