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Information on EC 4.99.1.3 - sirohydrochlorin cobaltochelatase
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4.99.1.3 sirohydrochlorin cobaltochelataseIUBMB Comments
This enzyme, which forms part of the anaerobic (early cobalt insertion) cobalamin biosynthesis pathway, is an ATP-independent type II chelatase. Two distinct forms are known - a primordial form named CbiX, which is most common in archaea, and a strictly bacterial form named CbiK. See EC 6.6.1.2, cobaltochelatase, for the cobaltochelatase that participates in the aerobic cobalamin biosynthesis pathway.
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Word Map
- 4.99.1.3
- protoporphyrin
- megaterium
- typhimurium
- chlorophyll
- precorrin-2
- uroporphyrinogen
- tetrapyrrole
-
primordial
-
sirohaem
-
dehydrogenation
-
histidine-rich
- epr
-
atp-independent
-
ferrochelation
The enzyme appears in viruses and cellular organisms
Synonyms
cobalt chelatase, cbixl, cbixs, sirohydrochlorin-ferrochelatase, sirohydrochlorin cobaltochelatase, anaerobic cobalt chelatase, archaeal cobaltochelatase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
anaerobic cobalt chelatase
archaeal cobaltochelatase
CbiK
CbiKc
CbiKp
CbiX
CbiX0H
CbiXL
CbiXS
cobalt chelatase
cobaltochelatase
sirohydrochlorin cobalt-lyase
sirohydrochlorin cobaltochelatase
sirohydrochlorin-ferrochelatase
additional information
-
the enzyme belongs to the AAA+ superfamily of proteins
anaerobic cobalt chelatase
-
-
-
-
anaerobic cobalt chelatase
-
-
CbiK
-
-
-
-
CbiX
-
-
-
-
CbiXS
-
-
-
-
cobaltochelatase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cobalt-sirohydrochlorin + 2 H+ = sirohydrochlorin + Co2+
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
cobalt-sirohydrochlorin cobalt-lyase (sirohydrochlorin-forming)
This enzyme, which forms part of the anaerobic (early cobalt insertion) cobalamin biosynthesis pathway, is an ATP-independent type II chelatase. Two distinct forms are known - a primordial form named CbiX, which is most common in archaea, and a strictly bacterial form named CbiK. See EC 6.6.1.2, cobaltochelatase, for the cobaltochelatase that participates in the aerobic cobalamin biosynthesis pathway.
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CAS REGISTRY NUMBER
COMMENTARY
81295-49-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
precorrin-2 + Co2+
cobalt-precorrin-2 + H+
-
-
-
?
ATP + sirohydrochlorin + Co2+
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Co2+
ADP + phosphate + Co(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
ATP + sirohydrochlorin + Fe2+
ADP + phosphate + Fe(II)-sirohydrochlorin + H+
-
-
-
?
precorrin-2 + Co2+
Co-precorrin-2 + H+
-
-
-
?
precorrin-2 + Co2+
Co-precorrin-2 + H+
-
tetrapyrrole biosynthesis, formation of tetrapyrrole cofactors
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
-
involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
a step involved in the vitamin B12 biosynthesis
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
a step involved in the vitamin B12 biosynthesis
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanobacter thermoautotrophicum
-
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanobacter thermoautotrophicum
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanosarcina barkeri Fusaro / DSM 804
-
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanosarcina barkeri Fusaro / DSM 804
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Priestia megaterium DSM 509
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin and siroheme biosynthesis,cbiK is able to substitute for cysG
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin branched biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent pathway
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent pathway
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent route, CbiK is a biglobal enzyme containing 2 a/b domains, which generate an active site with a deep rectangular cleft at their interface
-
?
sirohydrochlorin + Fe2+
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Fe2+
iron-sirohydrochlorin + 2 H+
both CbiKP and DELTA28CbiKP insert cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Fe2+
iron-sirohydrochlorin + 2 H+
the enzyme inserts cobalt and iron into sirohydrochlorin with specific activity with iron lower than that measured with cobalt
-
-
?
sirohydrochlorin + Fe2+
siroheme + H+
Methanobacter thermoautotrophicum
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
sirohydrochlorin + Fe2+
siroheme + H+
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
sirohydrochlorin + Fe2+
siroheme + H+
Methanosarcina barkeri Fusaro / DSM 804
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
additional information
?
-
-
CbiK fused together with CbiL as a multifunctional protein
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
Methanobacter thermoautotrophicum
-
precorrin-2 is no substrate
-
?
additional information
?
-
Methanosarcina barkeri Fusaro / DSM 804
-
precorrin-2 is no substrate
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
precorrin-2 + Co2+
Co-precorrin-2 + H+
-
tetrapyrrole biosynthesis, formation of tetrapyrrole cofactors
-
?
sirohydrochlorin + Fe2+
iron-sirohydrochlorin + 2 H+
-
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
-
involved in the formation of a octahedrally co-ordinated cobalt ion. In the oxygen-dependent cobalamin, i.e. vitamin B12, biosynthetic pathway, cobalt is inserted into a ring-contracted tetrapyrrole called hydrogenobyrinic acid a,c-diamide, HBAD, by a cobaltochelatase that is constituted by three subunits, CobN, CobS and CobT
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
a step involved in the vitamin B12 biosynthesis
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + 2 H+
a step involved in the vitamin B12 biosynthesis
-
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanobacter thermoautotrophicum
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Methanosarcina barkeri Fusaro / DSM 804
-
cobalamin biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
Priestia megaterium DSM 509
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin and siroheme biosynthesis,cbiK is able to substitute for cysG
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
cobalamin branched biosynthetic pathway
-
?
sirohydrochlorin + Co2+
cobalt-sirohydrochlorin + H+
-
biosynthesis of vitamin B12
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent pathway
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent pathway
-
?
sirohydrochlorin + Co2+
cobaltsirohydrochlorin + H+
-
cobalamin biosynthesis, oxygen-independent route, CbiK is a biglobal enzyme containing 2 a/b domains, which generate an active site with a deep rectangular cleft at their interface
-
?
sirohydrochlorin + Fe2+
siroheme + H+
Methanobacter thermoautotrophicum
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
sirohydrochlorin + Fe2+
siroheme + H+
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
sirohydrochlorin + Fe2+
siroheme + H+
Methanosarcina barkeri Fusaro / DSM 804
-
CbiXS can act as a ferrochelatase in the biosynthesis of siroheme in vivo
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKC is likely to be the enzyme associated with cytoplasmic cobalamin biosynthesis
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
additional information
?
-
CbiKP is periplasmic located and possibly associated with an iron transport system
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Fe2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.004
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
